Background: Mycoplasma pneumoniae (MP) is the primary causative agent of community-acquired pneumonia, which has increasingly become resistant to macrolides, complicating treatment regimens, especially with the co-infection factor. Its worldwide prevalence has fluctuated due to the influence of the COVID-19 pandemic. The study investigated co-infection patterns in children diagnosed with Mycoplasma pneumoniaepneumonia (MPP). Methods: From June 2022 to December 2023, we retrospectively analyzed the clinical data for hospitalized children with Mycoplasma pneumoniaepneumonia in Wuhan, China. We collected data on age, sex, clinical information, and pathogenic results. We also collected sputum or bronchoalveolar lavage fluid (BALF) samples to test respiratory pathogens and macrolide resistance using targeted microbial next-generation sequencing (tNGS). We analyzed the data using SPSS. Results: The study involved 417 patients diagnosed with MPP, of whom 86.33% had co-infections. Co-infections were notably linked to lobar pneumonia, prominent imaging shadows and higher macrolide resistance rate. Key bacterial pathogens were Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, rhinoviruses, and human adenoviruses (HADV). In MPP cases, Candida albicans was the fungal pathogen related to co-infections. The co-infection with HADV and human bocavirus 1 (HBoV1) correlated with prolonged fever, whereas Bordetella pertussis was linked to prolonged cough. In contrast, Candida albicans exhibited a weaker association with diffuse large-area infiltration on chest imaging, and its co-infection was less likely to result in severe disease. Conclusion: These results offer valuable insight into Mycoplasma pneumoniaepneumonia in children, highlighting the impact of co-infections on the disease’s clinical outcomes.
Agness Nicholaus KanusyaAbdishakur Abdukadir MuseBibek Dhar ShresthaYouping Deng
Introduction: The misuse of antibiotics has driven the emergence of antimicrobial resistance, with certain bacterial species, including Klebsiella pneumoniae, initially susceptible to most antibiotics, now exhibiting resistance to multiple antimicrobial agents. The objective of this study was to investigate the distribution and antimicrobial resistance profiles of Klebsiella pneumoniae strains isolated at the Yaoundé Central Hospital. Methodology: The study was conducted over a period of 4 months (March 1st, 2023-July 1st, 2023) and involved 32 strains isolated from bacterial cultures performed on patients, regardless of sex. The different Klebsiella pneumoniae strains were isolated using conventional methods. Identification, antibiogram, and detection of resistance enzyme production were generated using the VITEK 2 system. The Carbapenem-resistant K.N.I.V.O. kit was used to detect carbapenemases. Data analysis was performed using EXCEL 2019 software. Results: Out of 196 samples collected from various biological products, 32 Klebsiella pneumoniae strains were isolated, representing 16.32% (196/32). Urine samples were most frequently affected, accounting for 53.125%. The emergency department was the most represented (40.63%) by these isolates. The mean age was 50 years, with a minimum of 20 years and a maximum of 80 years. The sex ratio was equal to 1. The identified strains were resistant to cefotaxime (78.13%), cefoxitin (62.50%), tobramycin (71.88%), gentamicin (56.25%), ofloxacin (81.25%), and cotrimoxazole (78.13%). 78.25% were ESBL producers. Three strains were resistant to carbapenems, accounting for 9.37%;one of which exhibited the NDM type. Conclusion: This study highlights the evolving bacterial resistance to antibiotics, which requires adequate measures through the strengthening of the Antimicrobial Resistance (AMR) program in Cameroon.
目的应用自动化酶免工作站替代人工检测肺炎链球菌IgG抗体,以提高检测效率。方法依据世界卫生组织(World Health Organization,WHO)肺炎链球菌荚膜多糖型特异性IgG抗体检测指导手册《Pn PS ELISA》,设置工作站试验运行流程;最大化布局工作站台面板位数以预置全部试验材料,从而提高样本检测通量和减少人工干预次数。重复检测血清PnG,验证工作站试验精密度;检测WHO肺炎链球菌校准血清盘12/278,验证工作站试验准确度;使用工作站与人工方法检测16份相同血清样本,比较方法一致性;以双列方法(WHO指导手册标准布板方法)和单列方法检测4份校准血清,比较单、双列检测结果一致性。结果通过21个板位的布局,单次可检测24份血清样本,仅需2次人工干预;血清PnG的32次检测结果变异系数(CV)低于15%;血清盘23个型别检测结果的14个型别抗体均达到75%校准血清的检测值,与理论值的误差不超过±40%,总准确度为80%。工作站与人工检测结果的一致性相关系数Pc为0.9967,工作站单列与双列检测结果的一致性相关系数为0.9998,均具有非常高的一致性。结论自动化酶免工作站具有较高的精密度和准确度,较大的检测通量,且节省人力,可用于肺炎链球菌抗体检测。单、双列对比结果为样本单列检测代替双列检测提供了实验依据。
This article discusses the findings presented by Zhang et al.They analyzed the risk factors and clinical characteristics associated with Klebsiella pneumoniae infection in patients with liver cirrhosis treated at a hospital in Beijing.In this article,we focus on the connection between chronic kidney disease and the intestinal microbiota,and propose microbiota transplantation as a potential treatment for this patient group.We also examine an intriguing phenomenon related to hepatic encephalopathy,and provide insights into the future research.
