以大米与小麦粒作为主要材料,设计4组培养基栽培双梭孢虫草(Cordyceps bifusispora),比较子实体干重。采用高效液相色谱法测定子实体中虫草菌素和腺苷含量,采用3,5-二硝基水杨酸(DNS)法测定多糖含量。结果表明:在室内自然条件下,依据子实体干重,双梭孢虫草较适宜的栽培培养基为小麦粒加营养液1(20 g葡萄糖,5 g蛋白胨,3 g KH2PO4,1.5 g MgSO4,5 mg VB1);子实体的虫草菌素含量(103.1±15.7-122.1±18.6μg/g)各实验组间无显著性差异;依据子实体腺苷含量,适宜栽培培养基为小麦粒加营养液2(10 g葡萄糖,10 g酵母膏,1 g CaCl2,0.5 g MgSO4,0.5 g ZnSO4,0.1 g VB1),为1337.5±75.2μg/g;依据子实体多糖含量,适宜栽培培养基为大米加营养液2,为251.1±0.7 mg/g。
In order to further improve the yield of VA and VE produced by a Paecilomyces marquandii strain, using PDA liquid medium as the basic medium, the effects of adding nitrogen source, pH value and Fe2+ on the VA and VE production were compared with an orthogonal experiment. The results showed that the VA yield was significantly increased when sodium nitrate was added as nitrogen source, pH value of medium was 6, but Fe2+ was not added; the addition of peptone into the basic medium as a nitrogen source contributed to the improvement of VE yield in mycelia; the PDA liquid medium, as a basic medium, was more conducive to the VA production by the Paecilomyces marquandii strain.