The author mainly uses the Galerkin approximation method and the iteration inequalities of the L-Maslov type index theory to study the properties of brake subharmonic solutions for the Hamiltonian systems z(t) = JVH(t, z(t)), where H(t, z) = 1/2(B(t)z, z) + H(t, z), B(t) is a semipositive symmetric continuous matrix and H is unbounded and not uniformly coercive. It is proved that when the positive integers j and k satisfy the certain conditions, there exists a jT-periodic nonconstant brake solution zj such that zj and zkj are distinct.