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广东省自然科学基金(S2011010001353)

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相关作者:周晓穗陈佳林王方海张梅李广宏更多>>
相关机构:中山大学更多>>
发文基金:国家自然科学基金广东省自然科学基金更多>>
相关领域:生物学农业科学更多>>

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Effects of no mating on the female adults and embryonic development of Schistocerca gregaria (Orthoptera: Acrididae)
2013年
The newly emerged adult females of Schistocerca gregaria were separated and kept in the rearing conditions without males to examine the detailed developmental characteristic of the no mating females and carefully observed the developmental progress of non-fertilised eggs laid by virgin females. The results showed that the females kept without males survived in average 43 d, i.e. 58% of the life span of mated controls, and laid correspondingly fewer egg pods. The size of egg batches, the weight of eggs, and their content of ecdysteroids were normal but the hatch rate was reduced to 18.27%. Blocks of early embryogenesis (58.43%) and hatching failures (23.29%) were the major causes of death. Moreover, most of the hatched insects died during the postembryonic development and only a few females reached the adult stage. All insects that developed until the penultimate or last larval instar or reached the adult stage were females.
WANG FangHaiSEHNAL Frantiek
关键词:直翅目孵化率降低蝗科
昆虫JHE基因结构及与甲基化相关的CpG O/E值分析
2014年
本研究选择西方蜜蜂Apis mellifera、黑腹果蝇Drosophila melanogaster、致倦库蚊Culex quinquefasciatus和家蚕Bombyx mori 4种昆虫为例,以降解保幼激素的特异性酯酶(juvenile hormone esterase,JHE)为研究对象,首先运用NCBI的Spidey在线软件将各昆虫的JHE基因与其相应的基因组序列作比对,分别确定各昆虫JHE基因的上游2000 bp的具体序列和外显子及内含子区域。发现西方蜜蜂JHE基因含有7个外显子和6个内含子,而其余三种昆虫的JHE基因均含有6个外显子和5个内含子。接着,分别统计基因各区域的CpG,G,C位点的占有量,并计算出CpG O/E值(CpG位点的实际值与期望值之间的比值),发现各区域CpG O/E平均值的大小依次为:基因上游2000 bp区>内含子区>外显子区,基因上游2000 bp区的CpG O/E平均值大于1.0,而第1到第4外显子的CpG O/E平均值均只有0.8左右。表明在昆虫JHE基因中,若有CpG甲基化位点发生,应主要发生在第1到第4外显子区域。
张梅周晓穗陈佳林梁士可李广宏王方海
关键词:DNA甲基化基因结构CPG外显子
Study on the improvement of MS-RDA and its application in Sogatella furcifera (Horvath)
<正>Methylation-sensitive representational difference analysis(MS-RDA)is suitable for screening and identificat...
ZHOU XiaoSuiCHEN JiaLinZHANG MeiWANG FangHai
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