Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used successfully to determine three kinds of bisphthalazinone phenyl ether aromatic polymers with 1,8,9-trihydroxyanthranene as matrix. The polymerization degree of the polymers were determined to be 1 to 17. The influence of deflection value used in MALDI-TOF MS method was discussed.
This paper reports for the first time an experiment designed in which synthesized α cyano ferulic acid used as a new matrix for the analysis of cytochrome c, trypsin, bovine serum albumin, β cyclodextrin, dextrans and DNA d(T) 18 . The results demonstrated that α cyano ferulic acid used as a new matrix could effectively desorb and ionize different kinds of samples, such as proteins and peptides, oligosaccharides, polysaccharides and oligonucleotides. In analysis of dextrans the new matrix gave better results when comparing with the normally used DHB. In analysis of DNA d(T) 18 , the sample could be desorbed and ionized more efficiently even without sample purification, which is normally needed when using 3 HPA as a matrix.
近年来,为了克服基体辅助激光解吸/电离质谱(m atrix-assisted laser desorption/ion ization m ass spectro-m etry,MALD I-MS)测定小相对分子质量物质(sm all relative molecu larm ass compounds,SMC)(<1 500 u)所存在的问题,采取了许多行之有效的方法。这些方法的使用进一步促进了MALD I-MS对SMC的定性、定量分析。本文对这些方法的优缺点及在MALD I-MS分析SMC中的应用进行了综述。
