Splenic erythroblasts were obtained from mice during the acute disease caused by anemia-inducing virus (FVA). They were cultured in a medium containing EPO, BSA and so on. We studied their biomechanical and hemorheological behavior after 12 h, 24 h and 48 h. The results showed obvious changes in their electrophoretic mobility, osmotic fragility, membrane fluidity and membrane viscoelastic properties with the development of erythroblast. The changes were associated with the interactions of the protein and lipid and the elements of the membrane.
Using the method of gene transfection with liposome, we obtained the mouse erythro-leukemia cell line MEL-TF19, which stably carries TFAR19, a novel apoptosis-related gene. The expression of TFAR19 was detected by Western blot. Growth curve and flow cytometry analysis showed that after being transfected with TFAR19 gene, the growth of MEL-TF19 is suppressed and its apoptosis is accelerated because of the serum deprivation. Our biorheological study indi-cated that in the apoptotic process, compared with MEL cells, MEL-TF19 cells exhibit larger os-motic fragility, lower cell surface charge density, increased elastic modulus K1 which is inversely proportional to cells?maximal deformation ability, obviously diminished surface viscosity m, with elastic modulus K2 having no distinct changes. The above results provided some bases for recog-nizing the function of TFAR19 completely from the viewpoint of biorheology.
