Objective:To observe the effects of Liuwei Dihuang Granule(六味地黄颗粒,LDG) for tonifying Kidney(Shen) on the outcomes of in vitro fertilization pre-embryo transfer(IVF-ET) of infertility women with Kidneyyin deficiency syndrome and to explore its mechanism by detecting the proteome expression in the follicular fluid.Methods:Sixty-six infertility patients of Kidney-yin deficiency syndrome who would undergo IVF-ET,were randomly assigned to a treatment group and a control group according to a random number table,33 cases in each group.Another 33 cases of non-Kidney-yin deficiency syndrome was taken as a syndrome-control group.Besides Western routine therapy,LOG was given 3 menstrual cycles before IVF to the treatment group,and a placebo granule to the control and syndrome-control groups.The scores of Kidney-yin deficiency symptoms(sore waist and knees,dry vagina,dysphoria with feverish sensation in the chest,palms and soles,etc.) were assessed,the number of retrieved oocytes,rates of high quality oocytes and embryos,fertility rate and clinical pregnancy rate were recorded,and the follicular fluid was collected on the day when the ovum was picked up,the drfferential protein expression was detected using two-dimensional gel electrophoresis,and then,matrix assisted laser desorption ionization time-of flight mass spectrometry(MALDI-TOF-MS) was applied to identify the proteins.Results:The syndrome score in the treatment group decreased significantly from 16.09 ±2.58 to 8.67 ±2.13,while it changed insignificantly in the control group,with a significant difference in the lowering score between the two groups(P<0.05);the high quality rates of oocytes and embryos and clinical pregnancy rate were all superior in the treatment group to the control group(82.29%vs78.08%,76.76%vs 68.79%,63.64%vs 36.36%,all P<0.05).The protein expression map from the follicular fluid showed that compared with the control group,33 differential protein expressions were found in the syndrome-control group,among which 18 were down-regulated,
目的:探讨超促排卵周期中血清雌激素(E2)水平的变化及其与妊娠的关系。方法:选择2011年1月至2012年10月在我中心行IVF-ET(包括ICSI)治疗的516例不孕患者,按妊娠结局将患者分为妊娠组与非妊娠组。回顾分析患者注射HCG日与取卵日的血清E2水平,计算两日E2差值(⊿E2)与取卵日E2值的比值,并对此比值与妊娠结局进行相关性分析。结果:在超促排卵周期中,妊娠组与非妊娠组患者的⊿E2与取卵日E2值的比值差异显著(1.45±0.89 vs 1.44±1.54,P<0.05)。此比值与妊娠率呈负相关(r=-0.824,P<0.05)。结论:在控制性超排卵治疗中,⊿E2与取卵日E2值的比值可作为预测助孕结局的指标,此比值越大,越不利于妊娠结局。
