为了对从猫粪便样品中分离的钩虫虫卵进行种类鉴定,本研究提取该虫卵基因组DNA,根据核糖体内转录间区1(ITS1)的保守序列设计钩虫属引物,通过PCR扩增、克隆、测序、相关软件分析,建立系统进化树,从分子水平对其进行种类鉴定。结果表明扩增出404 bp DNA片段,测序结果显示该片段包括309 bp ITS1和95 bp的5.8S rDNA,与NCBI中登录的序列进行比对以及采用Clustal X和MEGA5.1软件分析确定该钩虫为锡兰钩虫。这是我国首次建立钩虫的分子鉴定方法,也是40年来再次发现猫源锡兰钩虫,为锡兰钩虫分子生物学和分子流行病学研究奠定了基础。
To establish a sensitive and rapid detection method for Giardia lamblia from dogs,two specific primer pairs fo...
Li Jie,Wang Peiyuan,Zhang Ping,Liu Yuanjia,Guo Jianchao, Meng Xianglong,Li GuoQing~* College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642 China
In order to identify the genotype of Giardia lamblia from dogs in Foshan,Guangdong.Two pairs of primers GF1/GR...
LI Jie,Wang Peiyuan,Zhang Ping,Zhu Haibo,Guo Jianchao,Li Guoqing~* College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642 China
