BACKGROUND: It has been found that micro RNA-423-5p(mi R423-5p) is an oncogenic factor and frequently upregulated in gastric carcinoma. However,the involvement of mi R423-5p in hepatocellular carcinoma(HCC) has been rarely reported. The aim of this study was to assess whether mi R423-5p is aberrantly expressed in HCC tissues,and to characterize its roles in the cancerous biology of HCC.METHODS: HCC and corresponding nonmalignant tissues were obtained from 115 patients during liver transplantation to detect the expression level of mi R423-5p. The mi R423-5p mimic and inhibitor were transfected into LM3 cell line. Cell viability assay,cell cycle analysis,transwell invasion and migration experiments were used to evaluate the oncogenic role of mi R423-5p.RESULTS: mi R423-5p was significantly upregulated in HCC compared with nonmalignant tissues,and this upregulation was negatively associated with recurrence-free survival. For patients beyond the Milan criteria,low expression of mi R423-5p was correlated with better prognosis. Functional analysis showed that mi R423-5p enhanced the proliferative,invasive and migratory capacity of HCC cells.CONCLUSIONS: mi R423-5p contributed to the tumorigenesis and progression of HCC. It could be a new predictor in HCC patients beyond the Milan criteria and would help to improve patient outcomes and enlarge recipient pools of liver transplantation.
BACKGROUND:Recurrence of hepatocellular carcinoma(HCC) after liver transplantation(LT) remains one of the most common causes of poor long-term survival.However,the host genetic factors affecting increased risk of tumor recurrence after transplantation have not been thoroughly elucidated.The present study was designed to investigate the association of cytokine gene polymorphisms with the risk of tumor recurrence in LT patients for HCC.METHODS:Eleven single-nucleotide polymorphisms within the promoter regions of 7 cytokine genes,i.e.,the IL-1 family(IL-1α and IL-1β),IL-6,IL-8,IL-10,TNF-α,and TGF-β1,were genotyped in 93 HCC patients treated with LT using DNA sequencing.The association between these polymorphisms and the risk of tumor recurrence was evaluated while controlling confounding clinical variables.RESULTS:The genotype frequency of IL-10-1082 A/G in patients with and without recurrence of HCC was AA 83.3%,GA 16.7% and AA 97.6%,GA 2.4%,respectively.The association between IL-10-1082 GA and recurrence was significant(P=0.033).No other single-nucleotide polymorphism in the cytokine gene was found to be associated with recurrence.Kaplan-Meier survival curves showed that the homozygous AA patients had a significantly longer mean recurrence-free survival than heterozygous GA patients(23.5 vs 5.7 months,P=0.001).However,multivariate analysis failed to reveal that the GA genotype of IL-10-1082 A/G was an independent indicator of recurrence.CONCLUSIONS:This study suggests the lack of association of selected cytokine gene polymorphisms with HCC recurrence after LT in the Han Chinese population.The finding does not exclude the idea that other cytokine polymorphisms could act as candidate biomarkers of disease prognosis.
