（E）-2-（1-（2-Hydroxy-4-methoxyphenyl）ethylideneamino）-3-（4-hydroxyphenyl）methyl propionate （C22H27O6N, Mr = 401.45） has been synthesized by a condensation reaction of paeonol with tyrosin methyl ester hydrochloride, and its structure was determined by IR, NMR, HR MS and X-ray single-crystal diffraction. The crystal belongs to the monoclinic system, space group P21/c, with a = 11.91291（15）, b = 9.49947（12）, c = 19.8727（3） , β = 106.1104（15）°, V = 2160.60（5）3, Z = 4, Dc = 1.234 g/cm3, μ = 0.739 mm-1, F（000） = 856, R = 0.0466 and wR = 0.1461 for 3859 observed reflections with （Ⅰ〉 2σ（Ⅰ））. In the crystal structure, the title compound is constructed by a centrosymmetric dimmer unit composed of a pair of π-π stacking enantiomers, and such units are linked by intermolecular O（5）-H（5）…O（1） and intramolecular N（1）-H（1）…O（1） hydrogen bonds.
