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国家自然科学基金(30872788)

作品数:4 被引量:82H指数:3
相关作者:申阿东尹青琴孙琳申晨徐放更多>>
相关机构:首都医科大学附属北京儿童医院更多>>
发文基金:国家自然科学基金北京市卫生局青年科学研究资助项目更多>>
相关领域:医药卫生更多>>

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Xpert MTB/RIF试验在结核病诊断中的研究现状被引量:49
2012年
据世界卫生组织统计,2010年全球新发结核病约880万例,结核病患者总数约1200万例,耐多药结核病约65万例。
尹青琴申阿东
关键词:结核病诊断RIF结核病患者耐多药结核病耐药情况
Ag85B表位多肽对体外单核细胞增殖影响的研究
2012年
目的通过观察Ag85B不同表位多肽对人外周血单个核细胞(PBMC)增殖的影响,筛选出增殖活性较强的Ag85B表位多肽,为制备结核新疫苗提供理论基础。方法采用的实验方法为人工预测Ag85B的表位,并合成多肽。将不同表位多肽体外刺激人外周血单个核细胞,利用CCK-8法测定单核细胞同一时相的吸光值,计算各自的增殖指数,并与BCG、Ag85B抗原等作对比。结果①Ag85B表位多肽组的OD450值及增殖指数均高于阴性对照组,且高于传统抗原BCG;②5条多肽中,28aa多肽的OD450值及增殖指数最高,且显著高于BCG(POD450=0.001<0.01,P增殖指数=0.013<0.05),但低于PHA;③28aa多肽的OD450值及增殖指数明显低于Ag85B,差异有统计学意义(POD450=P增殖指数=0.000<0.01)。结论预测的Ag85B表位多肽抗原具有一定的免疫原性,其中28aa多肽可作为优势表位多肽,但是否可用于表位多肽疫苗候选抗原还需进一步研究。
申丹肖婧孙琳焦伟伟苗青申晨徐放吴喜蓉申阿东
关键词:AG85B人外周血单个核细胞增殖
不同国家γ干扰素释放试验临床应用指南的概述被引量:13
2011年
γ干扰素释放试验(interferon gamma release assays,IGRAs)是一种新型的快速诊断结核分枝杆菌感染的免疫学方法。目前已经有16个国家制定了IGRAs诊断结核分枝杆菌感染的应用指南。各国IGRAs应用指南主要针对以下情况进行制定的:成人活动性结核病、成人潜伏结核感染、医疗工作者潜伏结核感染、免疫抑制者结核分枝杆菌感染筛查、儿童结核分枝杆菌感染。不同国家对不同人群的IGRAs应用指南不尽相同,故我国应根据本国国情针对不同人群进行大样本研究以得出IGRAs诊断结核分枝杆菌感染的最科学、最有效的应用原则。
尹青琴申阿东
关键词:Γ干扰素释放试验潜伏结核感染结核病
IFN-γ release assay: a diagnostic assistance tool of tuberculin skin test in pediatric tuberculosis in China被引量:21
2010年
Background Prompt diagnosis of Mycobacterium tuberculosis (MTB) infection is an essential step in tuberculosis control and elimination. However, it is often difficult to accurately diagnose pediatric tuberculosis (TB). The tuberculin test (TST) may have a low specificity because of cross-reactivity with antigens present in Mycobacterium bovis bacillus Calmette-Guerin (BCG) and other mycobacteria, especially in China with a predominantly BCG-vaccinated population.Early-secreted antigenic target 6-kDa protein (ESAT-6) and culture filtrate protein 10 (CFP-10), stand out as suitable antigens that induce an interferon-gamma (IFN-γ) secreting, T-cell-mediated immune response to infection. While,considered the higher costs and complexity of the IFN-γ release assay (TSPOT), we aimed to evaluate the TSPOT and TST test in the clinical diagnosis of pediatric tuberculosis and to establish a diagnostic process suitable for China.Methods The sensitivity and specificity of the assay were evaluated in total seventy four children with active tuberculosis and fifty one nontuberculous children with other disease, and then the results were compared with TST.Logistic regression models were used to identify variables that were associated with positive results for each assay. The independent variables included sex, age, birth place, vaccination history, close contract with an active TB patient.Results The sensitivity of TSPOT was higher than TST in active TB children with or without BCG vaccination, as well as in children with culture-confirmed TB. But the difference was not significant statistically. Combining results of the TSPOT and TST improved the sensitivity to 94.6%. Agreement of the TST and TSPOT was low (77.0%, k=0.203) in active TB patients. The difference in specificity between TSPOT and TST test was statistically significant (94.1% vs.70.6%, P=0.006). Specificity of the two tests in patients without prior BCG vaccination history was similar (80.0% vs.60.0%). The concordance betw
SUN LinYAN Hui-minHU Ying-huiJIAO Wei-weiGU YiXIAO JingLI Hui-minJIAO An-xiaGUO Ya-jieSHEN A-dong
关键词:INTERFERON-ΓTUBERCULOSIS
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