BACKGROUND:The early diagnosis of hepatocellular carcinoma(HCC) is of great clinical desirable due to lack of ...
Meng Fang,MD,Yun-Peng Zhao,MD,Lun-Gen Lu,MD,Peng Qi,Fei-Guo Zhou,MD,Hao Wang,Kun Zhou,Shu-Han Sun,PhD,Cui-Ying Chen,PhD,Chun-Fang Gao,MD 1 Department of Laboratory Medicine,Eastern Hepatobiliary Hospital,Second Military Medical University, Shanghai,China 2 Department of Gastroenterology,Renji Hospital,Jiaotong University School of Medicine,Shanghai,China 3 Department of Laboratory Medicine,Changzheng Hospital,Second Military Medical University,Shanghai, China 4 Department of Genetics,Second Military Medical University,Shanghai,China 5 Department for Molecular Biomedical Research,Ghent University and Flanders Interuniversity Institute for Biotechnology(VIB),Ghent,Belgium.
目的:体外重组表达人肝再生增强因子(augmenter of liver regeneration,ALR)并进行功能研究。方法:全基因合成ALR序列并插入原核表达载体pET28a+,转化入BL21菌内进行诱导表达,表达产物经过纯化后利用MTT法观察表达产物对人肝细胞的刺激增殖活性;利用小鼠急性四氯化碳损伤模型观察表达产物的肝功能保护作用。结果:酶切鉴定及测序结果提示表达产物正确;纯化后表达产物具有明确的促进肝细胞增殖作用,并在中、低剂量具有降低小鼠急性化学性损伤后转氨酶水平的作用。结论:重组表达的ALR结构正确,具有促进肝细胞再生的作用。
