Objective:Cyclophilin A (Cyp A) is a ubiquitously distributed protein belonging to the immunophilin family and is recognized as the host cell receptor for the potent immunosuppressive
Object： To observe the dynamic changes of dopamine （DA） in the striatum with the process of duplicating cerebral ischemia and reperfusion （CIR） model on rats. And observe the effect of electroacupuncture （EA） on such changes. Methods： Using microdialysis sampling, high performance liquid chromatography （HPLC） detecting technology, dynamic sampling from experimental animals at multiple moments to observe the changes of dopamine in striatum with ischemia. And observe the effect of EA at Fengchi （GB 20） on such changes. Result： About the extracellular level of DA in normal group, sham-operation group and sham-operation ＋ EA group, no significant changes were observed during the measuring period. The extracellular level of DA peaked at 15-45 min after MCAO and 0-30 min （P〈0.05） after reperfusion. A rising trend was also observed following 120 min after reperfusion. After EA, the extracellular level of DA was significant lower than ischemia group following 90 min after reperfusion （P 〈0.05）. And there was no peak again. Conclusion： EA at Fenchi （GB 20） can regulate the disorder of DA. EA can improve the integrity neurological function. This may be one of the mechanisms of acupuncture treating the lesion of cerebral ischemia and reperfusion.
Objective： To investigate the possible mechanisms in acupuncture analgesia by interaction of δ-opioid receptor with neurotransmitter transport proteins or the Na^＋-K^＋ pump. Methods： Microinjection of respective heterologous cRNA into the Xenopus oocytes as a model system, and measurement of steady-state currents under two-electrode voltage clamp. Results： The co-expression of the 8-opioid receptor with GAT1, EAAC 1 or the sodium pump resulted in reducing activity of the respective transporter. Opioid receptor activation affected transporter activity in different ways： 1） GAT1 was further inhibited; 2） EAAC1 was stimulated; 3） Na^＋-K^＋ pump activity interfered with agonist sensitivity of DOR. Pump inhibition led to higher sensitivity for DPDPE. Conclusion： GABA transporter inhibition and glutamate transporter stimulation may counteract pain sensation by affecting the neurotransmitter concentration in the synaptic cleft and, therefore, may contribute synergistically to pain suppression by acupuncture. Sodium pump inhibition by endogenous ouabain may amplify these effects. These synergistic effects may be the molecular mechanism of inhibiting pain sense and/or acupuncture analgesia.
The system of meridians and acupoints in the traditional Chinese medication （TCM） builds up a complex network. We analyze the features and properties of the system of meridians and acupoints as a complex network based on knowledge of TCM and mathematics. The system shows some important features of complex networks such as the small-world effect and the scale-free property. A preliminary discussion on the robustness of the meridian system as a complex network, and the biomedical functions of such robustness is also given.
Objective： To study the clinical effects of acupuncture therapy for acute stroke. Methods： 150 patients suffered from acute stroke were randomly divided into two groups： drug group, acupuncture group. To apply routine drug treatment of Neurology for drug group： pisacetam 250mL, Salvia injection 20mL added into normal sodium 250mL for intravenous drip, once a day. For acupuncture group applied ＂calm the liver and suppress yang＂ acupuncture therapy beside drugs. Modified Scandinavian Stroke Scale （ SSS ） and activities of daily living （ADL） scale （Barthel Index） were used for evaluation, as well as biochemistry/hemorheology determination. Results： The scale showed significant difference as compared with the drug group, biochemistry index had non-significant difference, and hemorheological index had partly improved. Conclusion： The scale could synchronously reflect the clinical symptom and changes of patient with stroke.Acupuncture therapy has a significant improvement of acute stroke treatment.
Pulse diagnosis plays a vital role in Traditional Chinese Medicine(TCM).To extract effective and properfeatures from the wrist-pulse-signals is a crucial step for the recognition and classification of the pulsesignals.Although the analysis in the time domain is also very effective for a lot of pulse types(SHU et al.,2007),the frequency and time-frequency analysis are usually used in the literature(YAN et al.,2005;YUEet al.,2006)because the pulse signals are non-stationary and non-periodic time series in the pathologicalstates.In recent years,a new time-frequency analysis method,Hilbert-HUANG Transform proposed
