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国家自然科学基金(50678049)

作品数:2 被引量:9H指数:2
相关作者:赵阳国王爱杰于振国阚洪晶刘春爽更多>>
相关机构:哈尔滨工业大学更多>>
发文基金:国家自然科学基金教育部“新世纪优秀人才支持计划”中国博士后科学基金更多>>
相关领域:生物学环境科学与工程更多>>

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SSCP技术分析活性污泥微生物群落结构的条件优化及检验被引量:7
2008年
单链构象多态性(SSCP)技术是一种分析环境微生物遗传多态性的有效方法,具有快速、简便、灵敏等特点。但是,该技术用于分析复杂环境微生物群落结构时需要有针对性地优化操作条件。本研究针对聚丙烯酰胺凝胶电泳(PAGE)胶浓度、上样缓冲液中去离子甲酰胺浓度、电泳温度、电泳时间等确定了PCR-SSCP技术的优化条件,并以自养脱硫反硝化反应器启动期的活性污泥样品对此优化条件进行了检验,证明了16SrDNAV1-V3区为靶对象时,丙烯酰胺与甲叉的质量比49:1,质量分数12%的聚丙烯酰胺凝胶,上样缓冲液中去离子甲酰胺的体积分数1/3,在4℃,300V,电泳18h是最优的操作条件,可以获较理想的SSCP图谱。
王爱杰阚洪晶于振国任南琪刘春爽张运晴赵阳国
关键词:单链构象多态性微生物群落
Microbial community structure in different wastewater treatment processes characterized by single-strand conformation polymorphism (SSCP) technique被引量:2
2008年
In order to investigate microbial community structures in different wastewater treatment processes and understand the relationship between the structures and the status of processes,the microbial community diversity,variety and distribution in five wastewater treatment pro cesses were studied by a culture-independent genetic fingerprinting technique single-strand conformation poly-morphism(SSCP).The five processes included denitrifying and phosphate-removal system(diminished N),Chinese traditional medicine wastewater treatment system(P),beer wastewater treatment system(W),fermentative biohydrogen-producing system(H),and sulfate-reduction system(S).The results indicated that the microbial community profiles in the wastewater bioreactors with the uniform status were very similar.The diversity of microbial populations was correlated with the complexity of organic contaminants in wastewater.Chinese traditional medicine wastewater contained more complex organic components;hence,the population diversity was higher than that of simple nutrient bioreactors fed with molasses wastewater.Compared with the strain bands in a simulated community,the relative proportion of some functional microbial populations in bioreactors was not dom-inant.Fermentative biohydrogen producer Ethanoligenens harbinense in the better condition bioreactor had only a 5% band density,and the Desulfovibrio sp.in the sulfate-reducing bioreactor had less than 1.5%band density.The SSCP profiles could identify the difference in microbial community structures in wastewater treatment processes,monitor some of the functional microbes in these processes,and consequently provide useful guidance for improving their efficiency.
Yangguo ZHAOAijie WANGNanqi RENYan ZHAO
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