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作品数:5 被引量:48H指数:4
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发文基金:国家自然科学基金浙江省自然科学基金浙江省大学生科技创新活动计划(新苗人才计划)项目更多>>
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多目标优化与多属性决策在补阳还五汤总苷提取中的应用
以补阳还五汤总苷提取工艺为示范,探讨中药提取工艺的多目标优化与多属性决策方法。采用BP人工神经网络建立提取工艺的多目标优化模型,依据划分网格进行非支配排序的算法求解模型,得出相应空间内Pareto解,使用多属性决策的支持...
盖玉权何昱张宇燕杨洁红周惠芬万海同
关键词:中药提取支持向量机多目标优化多属性决策
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响应面分析法优化红花红色素提取工艺的研究
目的运用响应面分析法优化碱溶酸沉法与双水相体系法提取红花中红色素的工艺,并对两种提取方法进行比较。方法单因素试验选取两种方法的实验因素与水平;然后根据中心组合实验设计原理,以红花红色素提取率为响应值,采用三因素三水平的响...
何昱孙燕雯朱英韩进周惠芬项海
关键词:红花红色素响应面分析法碱溶酸沉法
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丹红注射液与羟基红花黄色素A对大鼠脑缺血再灌注后IL-1β、TNF-α和Caspase-3 mRNA表达的影响被引量:19
2016年
目的:观察丹红注射液和羟基红花黄色素A(HSYA)对大鼠脑缺血再灌注(I/R)后白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)和Caspase-3 mRNA表达的影响。方法:将SD大鼠随机分为假手术组、模型组、丹红注射液治疗组(分高、中、低3个剂量组)、HSYA治疗组。应用线栓法建立大鼠中动脉阻塞(MCAO)再灌注模型;利用实时荧光定量PCR技术分析I/R组织中IL-1β、TNF-α和Caspase-3 mRNA表达的变化,以β-actin基因作为内参,采用△△Ct法计算mRNA相对表达水平。结果:与假手术组比较,模型组中IL-1β、TNF-α和Caspase-3 mRNA表达水平显著上调(P<0.01),而丹红注射液各剂量组对上述基因的表达具有明显抑制作用,且抑制效果随剂量的增大而增加。另外使用HSYA(2mg/kg)治疗同样能明显抑制IL-1β、TNF-α和Caspase-3的mRNA表达水平。结论:丹红注射液和HSYA均能明显抑制大鼠I/R脑组织中IL-1β、TNF-α和Caspase-3基因的过度表达。
朱振洪黄小飞王玉周郡潘利峰万海同
关键词:丹红注射液红花羟基红花黄色素A脑缺血再灌注白细胞介素-1ΒCASPASE-3
Efficacy of Yangyin Yiqi Huoxue Granule (养阴益气活血颗粒) in Treatment of Ischemic Stroke Patients with Qi-Yin Deficiency and Blood Stasis Syndrome: A Randomized, Double-Blind, Multicenter, Phase-2 Clinical Trial被引量:4
2021年
Objective To evaluate the efficacy and safety of Yangyin Yiqi Huoxue Granule(养阴益气活血颗粒,YYHG)in the treatment of ischemic stroke(IS)patients with qi-yin deficiency and blood stasis syndrome(QYDBSS),and to explore its effective dosage.Methods The total of 288 patients were randomly assigned to the YYHG high-dose,YYHG low-dose,positive control(administered Xiaoshuantong Granule,XSTG,消栓通颗粒),or placebo control(administered inert granule)groups(72 cases per group)by software-drived competitive block randomization.The trial was conducted for a 28-day period,with a 180-day follow-up period.The primary outcome was the comprehensive curative evaluation,and secondary outcomes were the National Institute of Health Stroke Scale(NIHSS)score,Barthel activities of daily living(ADL)index score,the quality of life index(QLI)score,and the Chinese medicine syndrome(CMS)score.All analyses were done on an intention-to-treat basis.The clinical safety was also assessed.Results The total of 288 participants were recruited between June 1,2008 and September 30,2009,and 287 patients received intervention;the treatment groups were well balanced at baseline.The comprehensive cure rates of YYHG high-dose,low-dose,positive and placebo control groups were 63.38%,31.94%,36.11%and 6.14%,respectively;there was a statistical difference between the two groups(P<0.01),while the high-dose YYHG treatment group was significantly higher than the other 3 groups(P<0.01).The improvement of NIHSS,ADL,QLI and CMS scores of the YYHG high-dose and low-dose groups was significantly better than that of the positive control group and the placebo control group(P<0.05).In terms of improving the classification of the NIHSS scale and the assessment of the ADL scale,the YYHG high-dose group was significantly better than the other three groups(P<0.05),and the YYHG low-dose group was better than the placebo control group(P<0.01).At the same time,except for the QLI score,the high-dose group was better than the low-dose group(P<0.05).In terms of saf
WANG YuYANG Jie-hongWAN Hai-tongHE YuXU BinAl Chang-shanZHOU Hui-fenYU LiWAN Hao-fangBIE Xiao-dong
丹红注射液主要有效成分配伍的药动学与药效学研究
目的建立同时测定大鼠血浆中丹参素、原儿茶酸、香草酸、丹酚酸B和羟基红花黄色素A(HYSA)质量浓度的HPLC-DAD方法,采用大鼠局灶性脑缺血再灌注模型,研究丹红注射液主要有效成分正交配伍的药代动力学特征,并在此基础上建...
艾进超
关键词:丹红注射液脑缺血再灌注药代动力学药效动力学
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丹红有效成分配伍对缺氧损伤脑微血管内皮细胞的保护作用被引量:5
2014年
为研究丹红有效成分配伍对缺氧致原代培养的乳鼠脑微血管内皮细胞(r BMECs)损伤的保护作用。原代培养乳鼠脑微血管内皮细胞并对细胞进行缺氧4 h损伤模型的同时,丹红有效成分(原儿茶醛、丹酚酸B、羟基红花黄色素A、丹参素)配伍作用于r BMECs,MTT确定细胞非毒性剂量,采用比色法测定LDH,SOD活性和MDA水平,RT-PCR法检测ICAM-1,MMP-9,P53 mRNA表达,流式细胞术检测r BMECs细胞周期及早期凋亡的变化。丹红有效成分配伍1,2,3,7,8,9组方显著抑制缺氧细胞上清液中LDH的增加;1,2,4,6,7组方显著增强缺氧细胞内SOD活性;1,2,3,8,9组方显著降低MDA水平;1,2,6,7,9组方显著抑制缺氧诱导的r BMECs早期凋亡,缺氧后细胞P53 mRNA表达明显上调的后果是能引起G1期阻滞和促进细胞凋亡,证实P53基因的调节功能体现在G1期校正点的监测;1,2,5,6,7,8,9组方显著下调P53 mRNA表达;1,4,7,8,9组方显著下调ICAM-1 mRNA表达;1,3,6,9组方显著下调MMP-9 mRNA表达。丹红有效成分配伍对缺氧所致原代培养的r BMECs损伤具有明显的保护作用,其机制与增强细胞抗氧化能力,抑制炎症反应和细胞凋亡有关。为研究方剂配伍规律提供思路,指导临床用药。
周惠芬何昱张宇燕杨洁红赵涛付巍周鹏万海同
关键词:抗炎症
HPLC-DAD法与UV-VIS法对丹红注射液有效成分和有效部位的质量控制研究被引量:7
2013年
目的:建立同时测定丹红注射液中丹参素、原儿茶醛、咖啡酸、迷迭香酸、丹酚酸B和丹酚酸A含量的高效液相色谱-二极管阵列检测器(HPLC-DAD)方法,运用紫外-可见分光光度法(UV-VIS)检测丹红注射液中有效部位(总黄酮)的含量。方法:丹红注射液中6种成分的定量采用Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm);乙腈-0.1%磷酸水流动相;流速1.0mL/min;波长330nm、280nm;柱温30℃。有效部位以芦丁为对照品,UV-VIS法进行测定。结果:样品中6种成分良好分离,线性关系良好(R>0.999,n=6),平均加样回收率分别为98.98%,100.86%,100.44%,98.73%,101.35%,100.17%。10批丹红注射液每1mL含6种有效成分的总量>11.50mg;总黄酮>9.00mg。结论:该方法操作简单,结果准确,具有较好的重复性和稳定性,可为丹红注射液的质量控制提供参考。
黄翔何昱杨洁红张宇燕万海同赵涛付巍邢攀科
关键词:丹红注射液紫外-可见分光光度法丹参素原儿茶醛丹酚酸A
Effects of Danhong Injection(丹红注射液) and Its Main Components on Anticoagulation and Fibrinolysis in Cultured Vein Endothelial Cells被引量:14
2016年
Objective: To observe the effects of Danhong Injection (丹红注射液) and its main components, including daiclzein and hydroxysafflor yellow A (HSYA), on the anticoagulation, fibrinolysis, anti-apoptosis in hypoxia model of vein endothelial cells (VECs). Methods: VECs were prepared and were put in a hypoxia environment, which consisted of mixed gas of 95% N2 and 5% CO2 mixed gas, when reached confluent culture. Five groups used different treatments, including normal control group, hypoxia group, daiclzein group, HSYA group and Danhong Injection group. The VECs were identified by fluorescence double labeling methods. The morphology was observed by a phase contrast microscopy. The effects of Danhong Injection, daiclzein and HSYA on 6 keto prostaglandin F1α (6-keto-PGF1 α) level was measured by the method of radioimmunoassay (RIA). Superoxide dismutase (SOD) activity was tested by water soluble tetrazolium salt. The content of malondialdehyde (MDA) was measured by thiobarbituric acid. The activities of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor (PAl) were measured by the method of chromogenic substrate. The contents of endothelin (ET) and nitric oxide (NO) were detected by non-equilibrium RIA and enzyme- linked immunosorbent assay. Cells apoptosis rate was determined by flow cytometry. Results: Compared with the normal control group, the floating cells number, PAl activity, ET and MDA contents, and cells apoptosis rate in the culture solution of hypoxia group were all significantly increased, whereas the 6-keto-PGF1 α and NO contents, and t-PA and SOD activities were decreased significantly (P〈0.01). Compared with the hypoxia group, Danhong Injection markedly increased the 6-keto-PGF1 α content and SOD activity, regulated PAl and t-PA activities, ET and NO contents, and decreased MDA content and cells apoptosis rate (P〈0.05 or P〈0.01). Conclusions: Danhong Injection and its main components played an important
张宇燕周惠芬杨洁红何昱陈小强Katsuyoshi Nishinari万浩芳万海同
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