Based on the floating reference theory, a new method for extracting the net analyte signal （NAS） is proposed. The noise background subspace is spanned by spectra at the floating radial reference point, and then, the spectra at the measurement point are projected on the subspace. Thereafter, the glucose concentrations in intralipid solutions are investigated through Monte Carlo simulation and experiments, and the partial least squares （PLS） models with and without NAS analysis are built. The root mean square errors of calibration and prediction reach to 28.87% and 27.33%, respectively. The results confirm the existence of information induced by glucose concentration variations as well as the validity of the floating reference theory.
A differential optical absorption spectroscopy (DOAS) method is presented and used for the air pollutant detection. The novel measurement frame employs a Cassegrain telescope and a combined fiber bundle. The emitting and receiving fibers are bundled together at one port. The common port is placed at the focus of the Cassegrain telescope. The total length of the prototype is reduced to about 1/2 of the traditional one. Air pollutants of SO2, NO2 and O3 are monitored by the prototype, and the concentrations are inverted. Results show that the correlation coefficients are 0.9490, 0.9614 and 0.9301, respectively. And there is a good consistency between the measured results and the reference data.
