建立了基于QuEChERS（quick，easy，cheap，effective，ragged and safe）-HPLC测定油炸食品中丙烯酰胺含量的方法。该方法的优势在于：改进的QuEChERS前处理方法中没有引入有机溶剂而直接采用纯水消除样品基底的影响。研究中，使用纯水超声提取丙烯酰胺，丙基乙二胺（PSA）吸附剂分散净化提取液，乙腈和水（体积比1：99）作为流动相，水系C18色谱柱分离，208nm紫外波长条件下检测丙烯酰胺的含量。结果表明，丙烯酰胺在0.020～0.800 μg／g浓度范围内与出峰面积呈线性关系，相关系数r=0.9994；检测限为0.005 μg／g；加标回收率为83.96％～89.43％，相对标准偏差（RSD）为2.13％～5.87％。该方法具有操作简单、快速、可靠和灵敏度高等特点，成功应用于方便面等油炸食品中丙烯酰胺含量的测定。
Trans-sobrerol （Sob） and 8-p-menthen-1,2-diol （Limo-diol） are the primary products in the atmospheric oxidation of α-pinene and limonene, respectively. Because of their low volatility, they associate more likely to the liquid particles in the atmosphere, where they are subject to the aqueous phase oxidation by the atmospheric oxidants. In this work, through experimental and theoretical study, we first provide the rate constants of Sob and Limo-diol reacting with hydroxyl radical （.OH） in aqueous solution at room temperature of 3044-3 K and 1 atm pressure, which are （3.05±0.5）×10 9 and （4.57±0.2）×10 9 L/（mol.s）, respectively. Quantum chemistry calculations have also been employed to demonstrate the solvent effect on the rate constants in aqueous phase and the calculated results agree well with the measurements. Some reaction products have been identified based on liquid chromatography combined with mass spectroscopy and theoretical calculations.
