To clarify the taxonomic status of Phlebotomus chinensb, the morphological characters of Ph. chinensis from Shaanxi, Henan, Sichuan and Gansu Provinces, and Ph. wui and Ph. longiductus from Xinjiang were examined and the rDNA-ITS2 region and mtDNA Cyt b gene sequences were analyzed simultaneously. It was found that morphological variation was very low in Ph. chinensis among the different localities; the wings of adult samples distributed in high-altitude areas were merely longer. The rDNA-ITS2 and mtDNA Cyt b sequences had an intraspecific variability. The relationships among species in the genus Phlebotomus can be inferred by molecular markers. These results suggest that there is an obvious genetic divergence at the molecular level among the Ph. chinensis populations in China.