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中国博士后科学基金(32134006)

作品数:2 被引量:14H指数:2
相关作者:陈溪萍张延波刘冉陶陆阳秦正红更多>>
相关机构:苏州大学更多>>
发文基金:中国博士后科学基金国家自然科学基金更多>>
相关领域:医药卫生更多>>

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Electroencephalogram and evoked potential parameters examined in Chinese mild head injury patients for forensic medicine被引量:2
2006年
Objective To evaluate the usefulness of quantitative electroencephalogram (QEEG), flash visual evoked potential (F-VEP) and auditory brainstem responses (ABR) as indicators of general neurological status. Methods Comparison was conducted on healthy controls (N=30) and patients with brain concussion (N=60) within 24 h after traumatic brain injury. Follow-up study of patient group was completed with the same standard paradigm 3 months later. All participants were recorded in multi-modality related potential testing in both early and late concussion at the same clinical setting. Glasgow coma scale, CT scanning, and physical examinations of neuro-psychological function, optic and auditory nervous system were performed before electroencephalogram (EEG) and evoked potential (EEG-EP) testing. Any participants showed abnormal changes of clinical examinations were excluded from the study. Average power of frequency spectrum and power ratios were selected for QEEG testing, and latency and amplitude of F-VEP and ABR were recorded. Results Between patients and normal controls, the results indicated: (1) Highly significance (P 〈 0.01) in average power of α1 and power ratios of θ/α1, 0/α2, α1/α2 of EEG recording; (2) N70-P 100 amplitude of F-VEP in significant difference at early brain concussion; and (3) apparent prolongation of Ⅰ~Ⅲ inter-peak latency of ABR appeared in some individuals at early stage after concussion. The follow-up study showed that some patients with concussion were also afflicted with characteristic changes of EEG components for both increments of α1 average power and θ/α2 power ratio after 3 months recording. Conclusion EEG testing has been shown to be more effective and sensitive than evoked potential tests alone on detecting functional state of patients with mild traumatic brain injury (MTBI). Increments of α1 average power and θ/α2 power ratio are the sensitive EEG parameters to determining early concussion and evaluating outcome of
Xi-Ping CHENLu-Yang TAOAndrew CN CHEN
关键词:CONCUSSIONPATIENTSELECTROENCEPHALOGRAM
大鼠脑外伤后溶酶体酶Cathepsin-B和D的表达被引量:12
2006年
目的研究大鼠脑外伤后溶酶体酶cathepsin-B和-D是否被激活及其不同时段表达变化,阐述其与凋亡执行因子caspase-3表达的关系,并探讨对脑外伤诊断及形成时间的意义。方法采用自由落体打击法建立脑外伤动物模型,并对模型及对照样本进行免疫荧光、双标和激光共聚焦检测,结果用SPSS10.0软件处理。结果脑外伤后1hcathepsin-B表达即增加,4~8d达高峰,脑外伤后32d仍处于高表达水平;cathepsin-D的表达于脑外伤后12h增加,4~8d达高峰,32d的表达仍然高于12h的表达水平。脑外伤初期,cathepsin-B和-D阳性细胞与caspase-3阳性细胞重叠较少,脑外伤后6h开始增加,32d仍然有很多阳性细胞重叠。结论脑外伤后cathepsin-B和-D被激活,其激活在脑外伤早期可能抑制细胞凋亡执行因子caspase-3的激活,之后(6h后)则与caspase-3起协同作用,共同促进细胞死亡;cathepsin-B和-D表达的时程变化对于脑外伤的法医学诊断和中晚期的时间推断有参考意义。
张延波陈溪萍陶陆阳秦正红李生兴杨丽杨菊张运阁刘冉
关键词:溶酶体
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