To investigate the molecular basis of porcine heterosis, suppression subtractive hybridization （SSH） was performed to detect the differences in gene expression between porcine longissimus dorsi of Meishan X Large White （MS × LW） F1 hybrids and their parents Meishan pigs. An expression sequence tag （EST） differentially expressed was found, designated as ML556, which was homologous to a hypothetical protein HSPC117, from human hematopoietic stem/progenitor cells （HSPCs）, and the full-length cDNA of porcine HSPC117 was cloned using rapid amplification of cDNA ends （RACE） method. Translation of the mRNA transcript revealed an open reading frame （ORF） of 505 amino acid residues encoding a peroxisomal targeting signal （PTS） with theoretical molecular weight of 55 kDa. Alignment analysis revealed that the deduced protein sequence exhibit 98, 98, 98, 97, and 97% identity with that of cattle, human, dog, rat, and mouse, respectively. The tissue expression analysis indicated that the porcine HSPC117 gene is highly expressed in muscle, spleen, lung, kidney, uterus, ovary and testis, moderately expressed in fat, heart, and liver, and not expressed in stomach and small intestine. The possible role of porcine HSPC117 and its relationship with porcine heterosis were discussed.
XIE Hong-tao LEI Ming-gang XIONG Yuan-zhu DENG Chang-yan JIANG Si-wen LI Feng-e ZUO Bo XU De-quan
＜正＞Introduction MMPs are zinc-dependent endopeptidases that digest extracellular matrix（ECM） and contribute to...
X.Wu~(1,2) and Y.C.Pan~1 1.Department of Animal Science,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,P.R.China 2.Biotechnology Research Institute,Shanghai Academy of Agricultural Sciences,2901 Beidi road,Shanghai,201106,China
In order to elucidate the domestication history of Peking ducks, 190 blood samples from six Chinese indigenous duck breeds were collected with186 individualsgenotyped by 15 microsatellite markers. Both the FST and Nei’s standard genetic distances (Ds) from the microsatellite data indicated high genetic differentiation between Peking duck and other Chinese indigenous breeds. The haplotype network with mtDNA data showed that most of the Peking duck haplotypes were distinctly different from those of other domestic breeds. Although the H01 haplotype was shared by all domesticated duck breeds, Peking ducks displayed 12 specific domestic duck haplotypes, including four similar haplotypes H02, H04, H08 and H22, that formed a single haplogroup (A). Both H02 and H22 haplotypes were also shared by mallard and Peking ducks, indicating that Peking ducks originated from wild mallard ducks.
QU LuJiang, LIU Wei, YANG FangXi, HOU ZhuoCheng, ZHENG JiangXia, XU GuiYun & YANG Ning State Laboratory for Agrobiotechnology and Department of Animal Science and Technology, China Agricultural University, Beijing 100193, China
