您的位置: 专家智库 > >

国家自然科学基金(30670477)

作品数:9 被引量:21H指数:2
相关作者:麻彤辉姜勇于波那万里刘丽丹更多>>
相关机构:东北师范大学吉林大学中日联谊医院吉林大学更多>>
发文基金:国家自然科学基金吉林省杰出青年科学基金国家重点基础研究发展计划更多>>
相关领域:生物学医药卫生理学轻工技术与工程更多>>

文献类型

  • 9篇期刊文章
  • 1篇会议论文

领域

  • 7篇生物学
  • 3篇医药卫生
  • 1篇轻工技术与工...
  • 1篇理学

主题

  • 3篇蛋白
  • 3篇水通道
  • 3篇水通道蛋白
  • 3篇通道蛋白
  • 3篇AQUAPO...
  • 2篇突变
  • 2篇AQUAPO...
  • 2篇CFTR
  • 2篇CURCUM...
  • 2篇NPA
  • 1篇原人参二醇
  • 1篇神经内分泌调...
  • 1篇水功能
  • 1篇水通道蛋白-...
  • 1篇水通道蛋白4
  • 1篇天然化合物
  • 1篇突变发生
  • 1篇人参
  • 1篇人参二醇
  • 1篇纤维化

机构

  • 3篇东北师范大学
  • 1篇吉林大学
  • 1篇吉林大学中日...

作者

  • 3篇麻彤辉
  • 1篇姜勇
  • 1篇肖英红
  • 1篇刘军
  • 1篇张红国
  • 1篇王宇
  • 1篇辛伟红
  • 1篇张英为
  • 1篇杨红
  • 1篇刘丽丹
  • 1篇那万里
  • 1篇魏建闯
  • 1篇于波

传媒

  • 6篇Chemic...
  • 1篇高等学校化学...
  • 1篇科学通报
  • 1篇Scienc...

年份

  • 2篇2010
  • 4篇2008
  • 4篇2007
9 条 记 录,以下是 1-10
排序方式:
20(S)-原人参二醇促进CFTR氯离子通道开放被引量:6
2008年
利用氯离子通道细胞荧光测定模型对386种中药单体化合物进行筛选,发现20(S)-原人参二醇对依赖于cAMP的CFTR氯离子通道具有激活作用.20(S)-原人参二醇能够以剂量依赖的方式激活野生型CFTR氯离子通道,其激活作用通过更为可靠的氯离子通道短路电流测定系统得到证实.20(S)-原人参二醇对CFTR氯离子通道的激活效应具有作用迅速且可逆的特点.其在发挥激活作用时依赖于腺苷环化酶激动剂Forskolin的存在,单独与细胞孵育不提高细胞内cAMP的水平,表明对CFTR氯离子通道的激活作用是通过与CFTR直接结合实现的.该化合物对ΔF508-CFTR突变氯离子通道的开放也具有特征相似的激活作用.
刘军那万里辛伟红刘丽丹于波麻彤辉杨红
关键词:囊性纤维化突变天然化合物激活剂
Remarkably Decreased CD11b Positive Splenocytes in Aquaporin 3-Null Mice
2010年
Aquaporins(AQPs) are molecular water channels that play important physiological roles in fluid trans-porting organs. The expression and function of AQPs in the immune system are largely unknown. CD 11 (a-d)/CD18 integrins are adhesion molecules expressed on leukocytes, which play a critical role in leukocyte adhesion, migration and host defense. In the present study, we discovered the expression of aquaporin-3(AQP3) on spleen CD1 lb positive cells, and the content of CDllb positive splenocytes in aquaporin 3-null mice is significantly decreased. Further analysis suggested remarkably decreased monocyte/macrophage subpopulation and significantly decreased granulocyte subpopulation. It is the first report suggesting an important role of AQP in the development and maturation of imrnunocytes.
ZHU NaQIAO YingZHANG DiWANG Wen-juanLIU JiaLIU Tian-zhouMA Tong-hui
关键词:AQUAPORINSPLENOCYTE
Prokaryotic Expression,Purification,Antibody Production of a NH_2-Terminal Fragment of mCLCA3 Protein and Analysis of mClCA3 Protein Expression in Asthmatic Mouse Lung被引量:1
2007年
mCLCA3 is a member of calcium activated chloride channel(CACC) family that may play an important role in mucin packaging and secretion in asthmatic and cystic fibrosis lung. To study the protein structure and expression of mCLCA3 in asthmatic mouse lung, an N-terminal 269 amino acid peptide of mCLCA3 was expressed in E. coli, purified to homogeneity and rabbit polyclonal antibodies against this peptide were generated. Immunohistochemistry of asthmatic mouse lung using the antibody indicated exclusive mCLCA3 expression in mucin granules of goblet cells in airway surface and lumen, Immunoblot analysis of lavage fluid from asthmatic mouse lung revealed a single 90 kDa protein form of mClCA3. The results demonstrate that the 90 kDa N-terminal peptide, neither the flail-length protein nor the reported N-terminal 35 kDa cleaved form of mClCA3 is the major functional form involved in the packaging and exocytosis of mucin granules in asthmatic goblet cells.
HOU XiaWU Qing-tianZHANG Yu-pingZHU NaLIU Yan-liFENG Xue-chaoMA Tong-hui
关键词:PURIFICATIONASTHMA
水通道蛋白4在神经内分泌调控中的作用
水通道蛋白在哺乳动物已有13种被鉴定,且不同的水通道蛋白在多种组织中表达。水通道蛋白4(AQP4)是一种选择性水通透膜蛋白,在大脑和脊髓中广泛表达,特别在参与形成血脑屏障的星形胶质细胞周足处以及与脑脊液直接接触的室管膜和...
张红国张英为肖英红王宇魏建闯麻彤辉
文献传递
Natural Compound Curcumin Corrects the Gating Defect of G551DMutant Cystic Fibrosis Transmembrane Conductance Regulator
2008年
In the present study, we identified the natural compound curcumin to be an effective G551D-CFTR activator by cell-based fluorescent assay and electrophysiological measurement. We demonstrated that curcumin can restore the impaired chloride conductance of G551D mutant CFTR. The activity is rapid, reversible, and cAMP-dependent. Our study identified a new natural lead compound for the pharmacological therapy of cystic fibrosis caused by G551D mutation of CFTR.
HAO Li-mingLIU XinXU Li-naZHU NaLIN SenHOU Shu-guangZHOU NaSHI De-chengSHANG De-jingMA Tong-huiYANG Hong
关键词:CFTRMUTATIONACTIVATOR
Importance of NPA motifs in the expression and function of water channel aquaporin-1被引量:1
2007年
The asparagine-proline-alanine sequences (NPA motifs) are highly conserved in aquaporin water channel family. Crystallographic studies of AQP1 structure demonstrated that the two NPA motifs are in the narrow central constriction of the channel, serving to bind water molecules for selective and effi-cient water passage. To investigate the importance of the two NPA motifs in the structure, function and biogenesis of aquaporin water channels, we generated AQP1 mutations with NPA1 deletion, NPA2 de-letion and NPA1,2 double deletion. The coding sequences of the three mutated cDNAs were subcloned into the mammalian expression vector pcDNA3.1 to form expression plasmids. We established stably transfected CHO cell lines expressing these AQP1 mutants. Immunofluorescence indicated that all the three mutated AQP1 proteins are expressed normally on the plasma membrane of stably transfected CHO cells, suggesting that deletion of NPA motifs does not influence the expression and intracellular processing of AQP1. Functional analysis demonstrated that NPA1 or NPA2 deletion reduced AQP1 water permeability by 49.6% and 46.7%, respectively, while NPA1,2 double deletion had little effect on AQP1 water permeability. These results provide evidence that NPA motifs are important for water per-meation but not essential for the expression, intracellular processing and the basic structure of AQP1 water channel.
JIANG Yong MA TongHui
关键词:水通道蛋白-1突变发生
Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve被引量:2
2010年
We reported the expression and function of aquaglyceroporin AQP3 in mouse peripheral nervous system.AQP3 mRNA was identified in freshly isolated mouse sciatic nerve by RT-PCR.Immunofluorescence using AQP3 antibody localized the protein expression in the myelin sheathe of sciatic nerve fibers.Although primary morpholo-gical evaluation of sciatic nerves from AQP3-knockout and wildtype mice revealed no significant difference,biochemical analysis demonstrated remarkably decreased glycerol and ATP contents in freshly isolated AQP3-knockout sciatic nerve.The study indicates an important role of facilitated glycerol transport mechanism in peripheral nerve energy metabolism.
ZHANG DiGUO LeiZHU NaSU Wei-hengGUAN Xin-gangYI FeiHAO FengXIAO Ying-hongLIU JiaLIU Tian-zhouMA Tong-hui
关键词:AQUAPORINGLYCEROL
NPA motif在水通道蛋白AQP1表达和转水功能中的重要性被引量:8
2007年
水通道蛋白(AQP)序列中的两个天冬酰胺-脯氨酸-丙氨酸序列(NPA motif)是水通道蛋白家族中高度保守的特征性序列,其晶体结构研究显示,两个NPA motifs位于通道中心的狭窄部位,直接参与水分子的结合和选择性通过.为了进一步研究两个NPA motifs在水通道蛋白结构、功能和生源学(biogenesis)方面的重要性,通过点突变方法分别获得单独缺失NPA1或NPA2 motif以及同时缺失两个NPA motifs的3种水通道AQP1基因突变型.将3种AQP1突变cDNA分别亚克隆入真核表达载体pcDNA3.1,建立稳定转染3种AQP1基因突变型的CHO细胞系.AQP1免疫荧光分析显示,3种AQP1突变蛋白均能正常表达并定位于质膜,显示AQP1蛋白质表达和细胞内加工过程未受NPA motifs缺失的影响.分别对表达3种AQP1突变蛋白的CHO细胞进行质膜水通透性功能分析,并与表达野生型AQP1的CHO细胞进行比较,结果显示,缺失NPA1或NPA2引起AQP1转水功能下降49.6%和46.7%,而同时缺失两个NPA motifs的AQP1转水功能与野生型AQP1相似.上述结果显示,水通道AQP1中的NPA motifs在AQP1转水功能中发挥重要作用,但对于AQP1蛋白的表达、细胞内加工和通道基本结构的形成并不起关键作用.
姜勇麻彤辉
关键词:水通道蛋白NPAMOTIF
Natural Compound Curcumin—a Channel Potentiator Rather Than a Corrector of the Defective Intracellular Processing of △F508 Mutant Cystic Fibrosis Transmembrane Conductance Regulator被引量:1
2008年
Cystic fibrosis(CF) is a severe genetic disease caused by the gene mutation of the cystic fibrosis transmembrane conductance regulator(CFTR) chloride channel. The most common point mutation AF508, which leads to impaired intracellular processing and channel gating of CFTR, appears in about 90% CF patients. The natural compound curcumin was reported to correct the processing defect of AF508-CFTR and proposed as a potential therapeutic drug to cure CF. In the present study, we analyzed the effect of curcumin on AF508-CFTR and demonstrated that curcumin can restore the impaired chloride conductance of AF508 mutant CFTR. The activity is rapid, reversible and cAMP-dependent. However, we couldn't reproduce the previously reported correction of the defective membrane trafficking of AF508-CFTR by curcumin. Therefore, curcumin may not be a superior lead compound for developing anti-CF drugs.
LIU XinGUAN LiHE Cheng-yanZHANG Xiao-jingXU Li-naSHANG De-jingMA Tong-huiYANG Hong
关键词:CFTR
Reduced Bone Mineral Density and Bone Metabolism in Aquaporin-1 Knockout Mice被引量:2
2007年
An overt phenotype of aquaporin-1 knockout(AQP1 ko) mice is growth retardation, suggesting possible defects in bone development and metabolism. In the present study, we analyzed the bone mineral density( BMD), bone calcium and phosphorus contents, and bone metabolism in an AQP1 ko mouse model. The BMD of femurs in AQP1 ko mice was significantly lower than that of litter-matched wildtype mice as measured by dual energy X-ray absorptiometry. Consistently, the contents of bone total calcium and phosphorus were also significantly lower in AQP1 ko mice. The reduced BMD caused by AQP1 deficiency mainly affect male mice. Bone metabolic activity, as indicated by 99m^Tc-MDP absorption measurements, was remarkably reduced in AQP1 ko mice. These results provide the first evidence that AQP1 play an important role in bone structure and metabolism.
WU Qing-tianMA Qing-jieHE Cheng-yanWANG Cai-xiaGAO ShiHOU XiaMA Tong-hui
关键词:AQUAPORIN
共1页<1>
聚类工具0