A procedure on cryopreservation of shoot-tips of Eriobotrya japonica Lindl.had been studied in vitro by two steps vitrification.The effects of cold-hardening,preculture,cryoprotecten treatment,thawing and reculture on survival rate of the shoot-tips were tested.Optimal media for shoot-tip induction and propagation were 1/2 MS+1.0 mg·L-1 6-BA+0.5 mg·L-1NAA and MS+0.5 mg·L-16-BA+0.1 mg·L-1 NAA.Preculture for 30 min with 60% PVS2(30% glycerol+15% ethylene glycol+15% DMSO+0.4 mol·L-1 sucrose) and treatment for 50 min by PVS2 were suitable to shoot-tip cryopreservation.After cryopreservationsome shoot-tips couldregenerate plantlets,the regenerate rate was 46.8%.There was no difference in regenerating plantlets and tube plantlets not stored by cryopreservation.
