c-Jun dimerization protein JDP2 is a member of the AP-1 family that serves as a repressor in gene transcription. To study the role of JDP2 in neuroblastoma cells, we have established a stably trans- fected SH-SY5Y cell line that carries jdp2 gene. Our results showed that JDP2 could promote neuronal differentiation of SH-SY5Y cells. The SY5Y-JDP2 cells exhibited lower percentage of cells in S phase and retarded growth rate than its parental cells that were accompanied by a higher expression of p21 and an intense reduction of the cyclin D1. In conclusion, the inhibitory role of JDP2 on neuroblastoma cell growth could be critical for the cells to be withdrawn from cell cycle and to proceed differentiation.
With chromatin immunoprecipitation (ChIP) and promoter DNA microarray analyses (ChIP-on-chip), we analyzed the variations of acetylation on histone H3 in all-trans retinoic acid (RA) induced neuronal cell differentiation. Neuroblastoma SH-SY5Y cells were treated with RA for 24 h and the acetylation on histone H3 in the promoter region of the genes was detected. Results showed that, after treatment, the level of acetylation on histone H3 elevated in 597 genes in the genome, and reduced in the other 647 genes compared with those of the control. In summary, we have successfully adopted a high throughput technique to detect and analyze variations of acetylation of histone H3 in human genome at the early phage of RA induced neuronal differentiation of the SH-SY5Y cells.
FANG HongBo MI Yang WU NingHua ZHANG Ye SHEN YuFei
