Objective: To investigate the expression of survivingene and its significance in acute leukemia. Methods:The expression of surviving in 134 acute leukemiapatients and 4 leukemia cell lines was detected byRT-PCR and immunofluorescence analysis. Results: We detected survivin expression in 78 of 134 acute leukemia patients and all the cell lines but not in normal controls and anemia patients. Survivin gene expressioncorrelated with a lower white blood cell count, whichwas 11?09/L and 48?09/L in the positive and negative group respectively (P<0.01 by the Mann-Whitney test). In 55 cases of FAB M1/M2/M3, it was associated withleukemic cell maturation(P<0.01 by the Fisher test).Survivin expression was strongly related to survival time of acute leukemia patients (P<0.05). Conclusion: These data suggest that survivin expression may be considered as a new unfavorable prognostic factor for acuteleukemia due to its important role in apoptosisinhibition that influences disease outcome.
The role of bcl 2 in the pathogenesis of colorectal tumor were studied. The exp ression of bcl 2 in the colorectal carcinoma and incisional edge tissue of tumo r was detected by using SABC method. The results showed that the positive rate o f bcl 2 was 69.6 % in colorectal carcinoma and 47.6 % in the incisional edge ti ssue respectively, with the difference being very significant ( P =0.001). B cl 2 positive rate was associated with Dukes’ stage, but had nothing to do with histological classification. It was concluded that bcl 2 might play a signific ant role in the development of colorectal carcinoma.
