We encapsulated vincristine into folic acid-conjugated PEGylated liposomes to improve the anti-tumor efficacy on multidrug resistant cancers.It was observed that the drug delivery system we constructed exhibited maximum cytotoxicity on KBv200 cells(multidrug resistant variant)compared with any other formulations.The semi-quantitative analysis of region of interest revealed that there was a great increase in area under curve(AUC)of a near-infrared fluorescein in solid tumors due to folic acid-mediated accumulation.Folic acid-conjugated PEGylated liposomes showed a significant tumor growth inhibiting effect in vitro and in vivo.TUNEL assay revealed that folic acid-conjugated PEGylated liposomes could induce cell apoptosis much more greatly than others.This study demonstrated that it had potential application prospective for the treatment of multidrug resistant cancer.
Chenyu WangLinglin FengXiangkun YangFei WangWeiyue Lu
The emerging nanotechnology-based drug delivery holds tremendous potential to deliver chemotherapeutic drugs for treatment of multidrug resistance(MDR) cancer.This drug delivery system could improve the pharmacokinetic behavior of antitumor drugs,deliver chemotherapeutic drugs to target sites,control release of drugs,and reduce the systemic toxicity of drugs in MDR cancer.This review addresses the use of nanotechnology to overcome MDR classified on the bases of the fundamental mechanisms of MDR and various approaches to deliver drugs for treatment of MDR cancer.
BU HuiHui,GAO Yu & LI YaPing Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China
Multidrug resistance(MDR)is an extremely complexed phenomenon in tumor chemotherapy and is one of the major obstacles for successful treatment.Discovery of RNA interference(RNAi)offers a new strategy with great potential to reverse MDR.Specific genes,which contribute to the emerging of MDR,can be silenced by RNAi.However,many natural barriers have to be overcome for efficient and safe delivery of siRNA.In recent years,the various kinds of nanocarriers,such as liposomes,cationic polymers,and inorganic materials,have been developed to deliver siRNA and show good results in reversing MDR.This review mainly summarizes the barriers in siRNA delivery and recent progress in designing nanotechnology-based siRNA delivery systems to overcome tumor MDR.
