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国家自然科学基金(20325518)

作品数:3 被引量:8H指数:1
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Chemiluminescence Immunoassay
<正>This year our group proposed a flow-injection CL immunoassay system based on a novel transparent immunoaffi...
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Al2O3/SnO2 Co-Nanoparticle Modified Grafted Collagen for Improving Thermal Stability and Infrared Emissivity
2007年
Al2O3/SnO2 co-nanoparticles were prepared with a modified sol-gel technique followed by a thermal treatment process. With these co-nanoparticles the grafted collagen-Al2O3/SnO2 nanocomposites were obtained using a supersonic dispersion method. X-ray diffraction, FT-IR analysis, transmission electron microscopy, TGA/DTA and infrared emissivity test were performed to characterize the resulting nanoparticles and nanocomposites, respectively. The Al2O3/SnO2 co-nanoparticles showed a narrow distribution of size between 20-40 nm and could be uniformly absorbed on the tri-helix scaffolds of the grafted collagen without any aggregation. The nanocomposites possessed better thermal stability and substantially lower infrared emissivity than the grafted collagen and Al2O3/SnO2 co-nanoparticles with an increase of degradation temperature from 39 to 210 ℃ and a decrease of infrared emissivity from 0.850 of the grafted collagen and 0.708 of the Al2O3/SnO2 co-nanoparticles to 0.424, which provided a potential application of the nanocomposites to areas such as photoelectronics.
曹勇周钰明单云鞠熀先薜学佳
关键词:NANOPARTICLENANOCOMPOSITE
Electrochemiluminescence Detection of Pharmaceuticals and Biomolecules with Capillary Electrophoresis or Nanotechnology
<正>In recent one year we developed a series of analytical methods based on ECL detection for pharmaceutical de...
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Electrochemical Biosensing Based on Nanoparticles assembly
<正>In recent years nanohiotechnology has been extensively used for bioanalysis Hue to the low cost and toxicit...
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季胺-辣根过氧化物酶-过氧化氢显色新体系及其在酶活性检测中的应用被引量:7
2007年
建立了光度法测定辣根过氧化物酶(HRP)活性的季胺-过氧化氢-HRP新体系,探讨了反应机理.该方法基于含KI的pH4.5PBS介质中,HRP催化H2O2氧化季胺[二(4–二甲氨基苯基)甲烷]的显色反应在462nm处的吸光度.吸光度与HRP活性呈线性关系.该可溶性的季胺比目前临床常用显色剂3,3',5,5'-四甲基联苯胺更稳定,克服了后者的缺点.在选定的实验条件下,测定HRP的线性范围为2.0×10-9~2.5×10-7g/mL,检出限为3×10-10g/mL.应用于HRP标记马抗人甲胎蛋白免疫标记物的测定,结果满意.该方法操作简便,灵敏度高,在临床上有较好的应用前景.
李建国刘颖鞠熀先
关键词:辣根过氧化物酶过氧化氢季胺
Effect of Enteral Nutrition Formula on Fat Absorption and Serum Free Fatty Acid Profiles in Rat with Short-Bowel Syndrome被引量:1
2006年
The effects of enteral nutrition containing long chain triglycerides (LCT) and medium chain triglycerides (MCT) or L-arginine (Arg) on fat absorbability, serum free fatty acid profiles and intestinal morphology in rats with short-bowel syndrome (SBS) were studied using gas chromatography. Twenty-eight Sprague-Dawley rats were randomly assigned to 4 groups: sham operation fed with LCT as control; 85% small bowel resection fed with LCT, MCT/LCT, and Arg/LCT, respectively. SBS rats showed a decrease of fat absorptivity. Enteral nutrition supplemented with MCT could increase fat absorptivity. L-Arginine enhanced enteral nutrition was associated with the elevation of fat absorptivity, possibly due to its enterotrophic effect on remnant small bowel mucosa. LCT group showed a significant deficiency of total free fatty acid and the decreased essential fatty acid content, which was improved in other two SBS groups.
周建农谭力王学浩鞠熀先
关键词:RAT
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