Agriculture soil in some areas of China is seriously damaged due to years of irrational farming practices.Soil-borne disease is a major problem of soil pollution,which affects yield and quality of agricultural products.Ecological remediation of soil is an effective way to solve this problem.In this study,Bacillus subtilis SY1 was successfully used to antagonist several normal fungal pathogens in eggplant.The growth and pathogenic tolerance of the host plant were improved after inoculation.In the seedling test,sprout tendency,accumulative germination percentage,sprout index,and vigour index of seeds increased 24%,24%,35%,and 64%,respectively.Inoculation also made the seedlings stronger and improved their plant-morphologic characters significantly.When infected by fungal pathogen,the activity of protective enzymes in inoculated seedlings improved,which helped lessening membrane damage by superoxide anion.
Yang ZongzhengLiu XinLiu ZhongPang JinzhaoQiu JinYang Wenyan
[目的]研究杨木APMP废液培养枯草芽孢杆菌SY1菌株(Bacillus subtilis)的适宜条件和转化液对番茄病原菌的拮抗作用。[方法]通过单因素实验对培养条件进行了初步优化;利用平板扩散法测定转化液对番茄病原菌的抑制作用。[结果]杨木APMP废液培养SY1菌株较适宜的条件为:pH值8.0,装液量75/250 m l,接种量5%,温度35℃;在此培养条件下SY1菌株的活芽孢数达3.96×108CFU/m l,废液COD转化率达59.52%。同时发酵液对供试的4种病原菌均有较强的抑菌作用。[结论]该研究为利用杨木APMP废液培养枯草芽孢杆菌的工业深层发酵提供了依据。
