To investigate the role of platelet membrane glycoprotein (GP) Ib/Ⅸ/Ⅴ complex and its subunit GP Ibα in patients with hemorrhagic thrombopathy (HT), the expressions of GP Ib/Ⅸ/Ⅴ complex and GP Ibα,defined as mean fluorescence intensity (MFI), were assessed by flow cytometry. The maximum aggregation of platelet was determined by turbidity method. These indicators were compared among 68 HT patients with the presenting complaint of hemorrhage, 33 well-controlled HT patients and 32 normal healthy subjects. The results showed that the MFI of GP Ib/Ⅸ /Ⅴ complex and GP Ibα was markedly lower in HT patients with current hemorrhage than that in the healthy subjects, with difference being statistically significant (P<0.05). There was no significant difference in the expressions of GP Ib/ Ⅸ/ Ⅴ complex and GP Ibα between well-controlled HT patients and normal healthy subjects (P>0.05). It was concluded that the expression of GP Ib/Ⅸ /Ⅴ complex, the receptor of thrombin and von Willebrand factor, was down-regulated in HT patients with current hemorrhage, which might result in the dysfunction of platelet aggregation and recurrence of HT.
Objective:To investigate the effect of Xiaoyu Zhixue Tablet(消瘀止血片,XYZXT) on the expression of platelet membrane glycoprotein(GP)Ⅰb/Ⅸ/Ⅴcomplex and GPⅠbαin patients with chronic renal failure(CRF)in early metaphase.Methods:Fifty-one patients with CRF in early metaphase(treated group)were treated with XYZXT,3 months as the course of treatment for 2 courses.The previous therapies remained unchanged.Flow cytometry was used to assess the expression of platelet GPⅠb/Ⅸ/Ⅴcomplex and GPⅠbαin patients with CRF,and turbidity method was used to determine the platelet maximum aggregation rate(MAR),meanwhile the renal function was measured.The final data were compared with those before the treatment,and with those in the normal control group(31 healthy subjects).Results:Compared with the normal control group,expressions of GPⅠb/Ⅸ/Ⅴcomplex and GPⅠbα,and platelet MAR in CRF patients were significantly lower(P=0.007,P=0.001,P=0.009)before the treatment;after the treatment with XYZXT, the above indexes in CRF patients were remarkably increased(P=0.033,P=0.026,P=0.045),but still lower than those in the normal control group,however,it was not statistically significant.Conclusion: (1)The expression of GPⅠb/Ⅸ/Ⅴcomplex in CRF patients of early metaphase was decreased, which lead to platelet aggregation dysfunction.This might be one of the reasons for the hemorrhagic trend in CRF.(2)XYZXT was able to upgrade expressions of GPⅠb/Ⅸ/Ⅴcomplex and GPⅠbαin CRF patients,improve platelet function and down-regulate platelet activation in patients with CRF.
