The ozonization of turpentine, α Pinene and β Pinene mixture in ethanol were studied by gas chromatography. The reactivity order is α Pinene> β Pinene and the ratio of kinetic rate constants , α Pinene k A and β Pinene k B is k A/ k B=7 0.
The pentadecanolide was biosynthesized in cyclohexane from 15-hydroxypentadecanoic acid, which as prepared from 15-tetracosenoic acid in Malania Olcifera Chum oil using lipase as catalyst. The effect of lipase activity on the lactonization was investigated. Lipases from fiften strains (13 strains of Candida and 2 strains of Penicillium) showed different catalytic effects to the synthesis reactions. The lipases from strains of Penicillium sp. and 4 strains of Candida sp. have catalytic lactonization activity, and the conversion ratio of substrate reached 88% by using lipase Candida sp.GXU01.
