[Objective]The aim was to research the effect of fast neutron irradiation on amylose content and amylopectin structure.[Method] The amylose content and amylopectin structure of the M4 plants of Thailand rice cultivar Jao Hom Nin irradiated with fast neutron at 13 Gy were evaluated and analyzed in this paper.[Result]The results showed that amylose content of rice could be changed by fast neutron irradiation,many rice mutants with reduced amylose content and many others with enhanced amylose content even some mutants with amylose content near to waxy rice could be isolated,but fast neutron irradiation almost had no effect on amylopectin structure of the samples.[Conclusion]The study provided a basis for breeding rice cultivar with different amylose content in order to meet with the taste hobby of different people and the further processing of diverse rice products via the irradiation of fast neutron.