您的位置: 专家智库 > >

张谦

作品数:29 被引量:136H指数:6
供职机构:北京林业大学更多>>
发文基金:国家自然科学基金国家教育部博士点基金国家转基因植物研究与产业化专项更多>>
相关领域:农业科学生物学文化科学自动化与计算机技术更多>>

文献类型

  • 15篇期刊文章
  • 8篇会议论文
  • 3篇专利
  • 2篇学位论文

领域

  • 20篇农业科学
  • 4篇生物学
  • 1篇自动化与计算...
  • 1篇文化科学

主题

  • 14篇基因
  • 11篇毛白杨
  • 11篇白杨
  • 5篇CPTI
  • 4篇杨树
  • 4篇甜杨
  • 3篇转基因
  • 3篇转基因杨
  • 3篇转基因杨树
  • 3篇林木
  • 3篇抗病
  • 2篇低温锻炼
  • 2篇电子克隆
  • 2篇烟草
  • 2篇胰蛋白酶抑制...
  • 2篇生物学
  • 2篇生物学技术
  • 2篇豇豆
  • 2篇豇豆胰蛋白酶...
  • 2篇豇豆胰蛋白酶...

机构

  • 27篇北京林业大学
  • 3篇华南农业大学
  • 2篇中华人民共和...
  • 1篇包头市林业局

作者

  • 28篇张谦
  • 22篇张志毅
  • 21篇林善枝
  • 14篇林元震
  • 3篇安新民
  • 3篇雷杨
  • 3篇郑会全
  • 2篇李海霞
  • 2篇张德强
  • 2篇刘文凤
  • 2篇刘婷婷
  • 2篇李琰
  • 2篇郭睆
  • 2篇王泽亮
  • 2篇郭海
  • 2篇雍军
  • 1篇吴定新
  • 1篇樊永明
  • 1篇罗磊
  • 1篇张学铭

传媒

  • 4篇Forest...
  • 3篇北京林业大学...
  • 3篇中国林学会林...
  • 2篇植物生理学通...
  • 2篇西北植物学报
  • 2篇植物生理与分...
  • 1篇西北农林科技...
  • 1篇分子植物育种
  • 1篇2006年全...

年份

  • 1篇2024
  • 1篇2012
  • 2篇2010
  • 2篇2009
  • 2篇2008
  • 2篇2007
  • 1篇2006
  • 3篇2005
  • 2篇2004
  • 1篇2003
  • 9篇2002
  • 2篇1997
29 条 记 录,以下是 1-10
排序方式:
甜杨6-磷酸葡萄糖脱氢酶在抗冻性低温诱导中的作用(英文)被引量:20
2005年
对-20℃低温锻炼及脱锻炼过程中甜杨 (Populus suaveolens)幼苗的G6PDH、SOD和POD 活性、MDA含量和半致死温度(LT50)进行了测 定和分析。结果发现,低温锻炼在一定程度上提 高了幼苗6-磷酸葡萄糖脱氢酶(G6PDH)、SOD和 POD活性,降低了MDA含量和幼苗半致死温度 (LT50)。另外,将幼苗放回常温(脱锻炼)2 d能引 起幼苗的G6PDH、SOD和POD活性的显著下降, 并使LT50和MDA含量的迅速回升。结果表明,低 温锻炼中G6PDH活性的增加有助于SOD和POD活 性的提高,进而对幼苗的LT50和MDA含量的降低 有明显的促进作用,G6PDH可能参与了SOD和 POD活性的调节和抗冻性的低温诱导。
林善枝张志毅刘文凤林元震张谦朱保庆
关键词:甜杨低温锻炼抗冻性半致死温度
山地苗木自压式滴灌装置
本实用新型为一种山地苗木自压式滴灌装置。其特点是在蓄水池和输水干管间安装一虹吸槽,槽中有虹吸管和控制虹吸管产生虹吸排水的浮球杠杆系统,使虹吸管在管径较大进水量较小的情况下也能产生虹吸现象,虹吸槽集小水为大水,产生间歇脉冲...
吴定新姚立红张谦
文献传递
毛白杨幼苗抗氧化系统对盐胁迫的响应
在3种不同浓度的NaCl(85 mmol L,170 mmol L,260 mmol L)胁迫下,对毛白杨幼苗的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性以及丙二醛(MDA)含量进行测定.结果发现,在NaCl胁迫...
林善枝林元震张志毅雍军张谦
关键词:毛白杨抗氧化系统盐胁迫
文献传递
Status and Advances of Molecular Genetic Improvement of Poplar Species in China被引量:2
2002年
Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many uses, easiness of tissue culture and small gene group that make them well suited as a model system for the application of genetic engineering in forest trees. In the last decade, much progress has been made in genetic improvement of poplar species in China. Modern biotechnology is an important tool for genetic improvement in forest trees, and its applications to genetic improvement in poplars, which covers genetic transformation, gene expression, construction of genetic linkage map, QTLs (quantitative trait loci) identification and molecular assisted selection are reviewed in this paper. At the same time, the existing problems and outlook about the application of modern biotechnology to genetic improvement in forest trees are also discussed.
张志毅林善枝张谦
关键词:POPLARQTLS
运用基因组和EST数据库进行电子克隆分离杨树功能基因的策略被引量:15
2007年
杨树是重要的造林绿化树种,也是主要的工业用材树种,还是高大林木的模式树种。进行杨树功能基因的挖掘、克隆和功能的研究,是当前杨树基因组学的首要任务之一,不仅可为杨树品种改良和基因资源的有效利用奠定基础,而且对探讨林木的生理及遗传特性分子机制也具有重要的理论意义。电子克隆是近年来伴随着基因组计划和EST计划发展起来的基因克隆新方法,具有成本低、速度快、技术要求低和针对性强等优点。丰富的杨树EST数据和公布的杨树基因组序列框架图,使得利用电子克隆技术开展杨树功能基因的分离和鉴定已经成为可能。本文阐述了电子克隆分离杨树功能基因的基本方法、相关的生物信息资源,并讨论了电子克隆技术存在的问题和未来发展。
林元震张志毅林善枝张谦刘纯鑫郭海
关键词:杨树功能基因电子克隆
Test of Insect-Resistance of Transgenic Poplar with CpTI Gene被引量:3
2002年
Both non-transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] and transgenic ones expressing cowpea trypsin inhibitor were cut at the base of the stem to produce auxoblasts, and used as source of leaves for insect feeding trials performed on 3 major insect species of poplar, the forest tent caterpillar ( Malacosoma disstria L.), gypsy moth ( Lymantria dispar L.) and willow moth ( Stilpnotia candida Staudinger). The height and basal diameter of trees were measured by the end of that year (2000). The results indicated that the growth elements of transgenic poplars were not interfered by the incorporation of the CpTI gene. Intriguingly, the height and basal diameter of the clone TG04 were much greater than that of the control. The transgenic foliage consumed by insects induced the increase of larval mortality, and decrease of larval wet weight gain, faecal output, pupal weight and egg deposition. Among them 3 transgenic clones, TG04, TG07 and TG71 received special attention for their outstanding insect resistance compared with other transgenic clones, which showed that the CpTI gene in them was expressed more actively and stably than in others.
张谦林善枝张志毅林元震
关键词:LEPIDOPTERA
Identification of Expression of CpTI Gene in Transgenic Poplars at Protein Level
2002年
The contents of total soluble protein and cowpea trypsin inhibitor (CpTI) in the browse and metaphylla of transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] transformed with CpTI gene were determined in order to study the products of CpTI gene expression at protein level. The results indicated that the amount of total soluble protein was greater in transgenic poplars than in non transgenic poplars, but was more in the metaphylla than in browse. The expression of CpTI gene resulted in an obvious increase in CpTI content, whereas CpTI was not detected in non transgenic poplars. It was found that there were high amount of total soluble protein and CpTI in 3 clones of TG07, TG04 and TG71 compared with other transgenic clones. In addition, the analysis of protein by SDS PAGE showed that a specific protein band of about 11.3?kD corresponding to the 80 amino acids encoded by the CpTI gene was observed in transgenic poplars on the gel of protein, which was not detected in non transgenic poplars.
林元震张谦林善枝张志毅
关键词:TRANSFORMATIONCPTI
Role of CaCl_(2) in Cold Acclimation-Induced Freezing Resistance of Populus tomentosa Cuttings被引量:2
2002年
Populus tomentosa cuttings were treated with 1mmol·L -1 , 5mmol·L -1 , 10mmol·L -1 or 15mmol·L -1 of CaCl 2 for 1\|7 d, respectively, for studying the effects of different concentrations of CaCl 2 on freezing resistance. Results indicated that 10?mmol·L -1 of CaCl 2 has greater effect than other concentrations on the enhancement of freezing resistance, and the optimum time of pretreatment was 5?d. In addition, cuttings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 of CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 05?mmol·L -1 of CaM antagonist CPZ or 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3 The changes in CaM and freezing resistance of all cuttings were investigated. The results showed that cold acclimation at -3℃ increased CaM content and decreased the minimum temperature for 100% survival. The CaCl 2 pretreatment enhanced the effect of cold acclimation and obviously increased CaM content and decreased the minimum temperature for 100% survival, but this effect was strongly inhibited by the EGTA, CPZ or LaCl 3 It is concluded that the effect of CaCl 2 on freezing resistance is associated with its concentration and time of pretreatment, Ca 2+ CaM may be involved in the induction of freezing resistance of the cuttings.
林善枝林元震张志毅雍军张谦
关键词:CAM
PID参数自整定调节器的研究被引量:5
1997年
该文对基于规则的调节器参数的自整定方法进行了研究,给出了仿真结果。
张谦
关键词:专家系统自整定仿真调节器
表达豇豆胰蛋白酶抑制剂转基因杨树的虫试试验
将转豇豆胰蛋白酶抑制剂基因的三倍体毛白杨回交杂种[(毛新杨*毛白杨)×毛白杨]]于春季萌发前截干以获取萌条。选用萌条上的树叶对三种主要杨树害虫:天幕毛虫(Malacosoma disstria)、舞毒蛾(Lymantri...
张谦林善枝张志毅林元震
关键词:毛白杨鳞翅目昆虫转基因
文献传递
共3页<123>
聚类工具0