[Method] This study aimed to assess the changes in grassland ecosystem Service values in the Three-River Headwaters Region of China, the source of the Yangtze, Yellow （Huang He） and Lantsang （Mekong） rivers. [Method] Biophysical values of four services were monetized within the region, including water regulation, air quality regulation, climate regulation and soil conservation. [Result] The total ESVs were 884.97×10^8 Yuan, 1 302.06×10^8 Yuan and 1 299.49×10^8 Yuan in 2000, 2005 and 2008, respectively. The amount of value per unit area experienced a steep increase from 2000 to 2005（18.10×10^4 Yuan/km2）, and then remained almost unchanged from 2005 to 2008 （-0.31×10^4 Yuan/km2）. The ESV tended to decline from the southeastern to the northwestern. ESV in the eastern and central part increased faster than that in the south-central and western part of the TRHR from 2000 to 2008. It could be seen that the ecosystem condition of grassland in the TRHR improved signifi- cantly over the study period of 2000-2008. [Conelusion] The results provided good information to assess the effectiveness of current ecological protection measures in the TRHR and support regional sustainable management policies.
