The extraction behavior of Fe（Ⅲ）, Cu（Ⅱ）, Co（Ⅱ）, Mn（Ⅱ）, Pb（Ⅱ）, Ni（Ⅱ） and Hg（Ⅱ） by microemulsion Triton X-100 (α-（4-（1,1,3,3-tetramethy（butyl）phenyl）-ω-hydroxy）poly（oxy-1,2-ethanediyl） in the presence of （NH4）2SO4 and complexing agents were investigated. The results indicated that the complexes of Fe（Ⅲ） with aluminon were almost completely extracted in HAc-NaAc buffered microemulsion while Cu（Ⅱ）, Co（Ⅱ）, Cd（Ⅱ）, Mn（Ⅱ） and Hg（Ⅱ） were not. Quantitative separation of Fe（Ⅲ）, Co（Ⅱ） and Mn（Ⅱ） were realized. The extraction mechanism by O/W microemulsion has been discussed.
The interaction of methylene blue(MB) and bovine serum albumin(BSA) is investigated in the SDS micelle system which is simulated as one kind of coexisted albumin. The interaction parameters of MB and BSA and simulated albumin such as partition coefficientκ、 normal binding free energyΔ G、 average binding number n are calculated. The results show that most of MB is in the form of monomer in SDS micelle systems； the main interaction of MB and BSA is of static electric and H-bind force,and that of MB and simulated albumin is only of static electric force.
