应用绵羊精子提取物体外激活绵羊体细胞核移植胚和山羊-绵羊异种核移植胚,比较了不同浓度的绵羊精子提取物对绵羊同种和异种重构胚的激活作用,并与传统的化学激活方法对重构胚的卵裂率、囊胚率和囊胚细胞数做了比较。结果表明,绵羊精子提取物可以有效地激活绵羊体细胞核移植胚和山羊-绵羊异种核移植胚,每一重构胚注射3 pL浓度为5 mg/mL的绵羊精子提取物,对同种和异种胚的发育效果均为最好,并显著高于其他试验组(P<0.05)。注射绵羊精子提取物对绵羊-山羊异种核移植胚的卵裂率、囊胚率和囊胚细胞数的影响,与离子霉素联合6-DAMP激活法差异不显著(P>0.05)。注射绵羊精子提取物对绵羊同种核移植胚的卵裂率和囊胚率的影响,与离子霉素联合6-DAMP激活法差异也不显著(P>0.05),但精子提取物注射法的绵羊核移植胚的囊胚细胞数显著(P<0.05)高于化学激活法的细胞数(72.4 vs 65.2)。
Lung of the somatic cell cloning goat "yuan-yuan" was observed with eyes,LM and TEM,the results are as follows: The left lung have two lobes and the right lung have four lobes.The fissures are incomplete which are connected with lobes by serosa.Many epithelial cells are full of osmotin.The respiratory bronchioles were lined with cuboid cells.The alveoli epithelium differentiated into flattened(type I cell) and cuboid(type II cell)epithelial cells.The capillary endothelium were pressed close to the alveolar epithelium.The bloodair barrier was composed of type I cell,basement membrane and endothelium.The osmiophilic lamellar bodies and ribosomes are seen abundantly in type II alveolar cells,rough endoplasmic reticulum pools expand,Mitochondria denatured,microvilli of the epithelial cells appear on the luminal surface.
