通过测定抗病性不同的小麦品种在接种小麦白粉病菌后钙调素含量的变化发现其存在差异。如抗病品种钙调素含量高峰出现在接种后 4 h,即寄主与白粉病菌接触阶段。而感病品种接种后24 h出现钙调素含量高峰。比较测定了不同的钙调素抑制剂对小麦品种抗白粉性的影响,发现 10m m ol/L TFP和 5 m m ol/L CPZ处理使抗病品种变为高度感病,50 m m ol/L CPZ处理使小麦感染白粉菌后反应型下降,表明钙调素与小麦抗白粉性有关。
Banana bunchy top virus disease (BBTD) is a disastrous disease in bananas, and it is spreading in the world (including China) by the banana bunchy top virus(BBTV). At present, virus\|free plantlets are used to prevent BBTD in banana production, therefore, it is very important to establish a method to detect BBTV quickly, sensitively and specifically. ELISA is now popularly used to detect BBTV. The sensitivity of this method is not high enough, and needs specific antiserum, otherwise, pseudo\|positive results often occur. According to DNA coding sequences of component Ⅲ,Ⅳ and Ⅰ of BBTV isolates from Zhangzhou, China, three pairs of primers are designed to establish a PCR method to specifically amplify parts of coding sequences of the BBTV coat protein, movement protein and replicase\|association. This method is also applicable to detect BBTV of bananas or cultured banana seedlings in other regions.
