Root rot disease is one of the most important diseases of Bupleurum chinense DC..Pure isolates from host tissues were obtained.The results of pathogenicity test,morphology observation and ITS sequence analysis indicated that the disease was caused by Fusarium oxysporum.
目的探讨聚合人脐带血红蛋白(PolyCHb)对动物心肌组织的影响。方法取18只健康雄性SD大鼠随机均分为假手术(Sham)组:大鼠麻醉后仅行股动、静脉插管,颈静脉及右侧颈总动脉插管;NaCl组及PolyCHb组:除同Sham组一样麻醉、插管后,分别输注生理盐水或PolyCHb(800 mg/kg);连续监测基础值、输注后0、10、20、30、60 min 3组大鼠的平均动脉压(MAP)、心率(HR)、左心室收缩末期压力(pes)、左心室舒张末期压力(ped)、室内压最大上升速率(dp/dt max)、室内压最大下降速率(dp/dt min)、等容舒张时间常数(Tau)。另取18只大鼠同样分作3组,麻醉、插管后按分组做相应处理,取输后1、3、6 h血样于EDTA抗凝管中,离心取血浆,测定肌酸激酶同工酶(CK-MB)及肌钙蛋白Ⅰ(cTnⅠ)含量,6 h时处死大鼠,取心脏做病理学检测。结果 PolyCHb组、 NaCl组与sham组比较:1)术(输)后0—60 min MAP(mmHg)及pes(mmHg)持续升高,至输注后60 min时,分别为128.50±7.26 vs 115.67±4.72 vs 106.5±14.84(P<0.05)与148.30±18.16 vs 122.83±13.57 vs 122.07±19.00(P<0.05);术(输)后HR(次/min):0 min时分别为302.83±20.76vs 371.00±43.36 vs 387.50±47.27 (P<0.05), 60 min时分别为391.50±34.26 vs 416.83±33.47 vs 431.50±33.76 (P>0.05); ped(mmHg):60 min时分别为11.34±11.58 vs 6.83±4.79 vs 8.14±4.25(P>0.05);2)dp/dt max(mmHg/s)与dp/dt min(mmHg/s)术(输)注0 min分别为6 530.50±418.63 vs 8 341.50±1 095.00 vs 8 242.17±1 579.38与5 352.83±449.02 vs 7 687.00±1 111.87 vs 7 799.50±2 246.39(P<0.05),至术(输)后60 min分别为8 139.00±758.26 vs 10 083.33±1 256.59 vs 10 032.17±1 779.30与7 457.33±915.62 vs 9 192.00±892.90 vs 9 069.50±2 104.49(P<0.05);3)Tau术(输)后0 min分别为17.01±3.72 vs 12.69±3.32 vs 12.57±2.07(P<0.05);4)CK-MB(pg/mL)术(输)后1、3 h术(输)后1、3 h时分别为63.41±13.15 vs 49.67±20.10 vs 38.20±6.02与101.88±28.08 vs 37.80±18.21 vs 37.94±10.71(P<0.05);5)cTnI(pg/mL)术(输)后3 h分别为14.61±7.03 vs 5.32±2.96 vs 5.72±2.
