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周贝贝

作品数:20 被引量:138H指数:7
供职机构:北京市农林科学院林业果树研究所更多>>
发文基金:国家林业公益性行业科研专项国家科技支撑计划国家自然科学基金更多>>
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20 条 记 录,以下是 1-10
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Correlation Analysis between Quality Characteristics and Fruit Mineral Element Contents in 'Fuji' Apples
2017年
[Objective] The aims were to explore the relationship between the contents of fruit mineral elements and quality features of the 'Fuji' apple, screen major mineral elements of the fruit affecting fruit quality features, and set up optimum proposals of fruit mineral elements for good fruit qualities, so as to provide a theoretical basis for the reasonable orchard soil and foliar fertilizer applications to increase fruit quality and reduce the physiological diseases related to the 'Fuji' apple. [Methods] The fruit mineral elements and quality indicators of 'Fuji" apples were in- vestigated and analyzed, which were collected from the 153 commercial apple or- chards of "Fuji' apple located in 51 counties. The variable importance for projection (VlP) of partial least squares regression (PLS) method was used to analyze the model effect and weight analysis impact of the fruit mineral element contents to fruit quality, screen out major factors of fruit mineral elements influencing the different fruit qualities, and set up the regression equation of the fruit qualities and major fruit mineral elements. Linear programming was used to obtain optimum proposals of the fruit mineral elements to achieve good 'Fuji' apple qualities. [Results] The mineral elements content and quality characteristics in "Fuji' apple fruit had great differences in the different produce regions in which the maximum content of nitro- gen, iron, zinc and boron in the 'Fuji' fruit were12.06, 6.17, 7.7, and 10.08 times greater than the minimum respectively, and the differences for titratable acid and the SSC/TA ratio were 2.33 and 2.16 times respectively. The correlation analysis between the fruit mineral element contents and qualities showed that the nitrogen content of fruit had a significantly negative correlation with the soluble solid content, SSC/TA ratio and red color area, while the calcium and iron contents in the fruit were in significantly positive correlation with the soluble solid content and SSC/TA ratio
张强李兴亮李民吉周贝贝张军科魏钦平
适合MSAP分析的核桃子叶DNA提取方法研究
核桃成熟子叶组织脂肪酸含量较高,组织培养后多糖多酚含量升高,而DNA中多糖等杂质的残留对酶切等影响较大.为获得适合MSAP 分析的高质量DNA,本研究以‘辽宁1号’核桃品种成熟子叶和经过组织培养的近成熟子叶为材料,以叶片...
周贝贝陈凌娜王伟马庆国宋晓波张俊佩裴东
关键词:核桃多糖MSAP
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‘富士’苹果品质与果实矿质元素含量的关联性分析被引量:30
2016年
【目的】探讨我国‘富士’苹果品质与果实矿质元素含量的关系,筛选影响果实品质特性的主要矿质元素,建立‘富士’苹果优质的果实矿质元素含量优化方案,为提高‘富士’果实品质、减少生理病害的果园合理土壤和叶面施肥等提供理论依据。【方法】2010—2011年对我国苹果产区51个县(市)153个乔砧‘富士’苹果园的果实矿质元素和品质指标进行调查分析,应用偏最小二乘回归变量投影重要性筛选影响果实品质因子的主要果实矿质元素和建立回归方程,线性规划方法求解‘富士’苹果优质的果实矿质元素含量优化方案。【结果】我国‘富士’苹果果实中不同矿质元素含量和品质差异较大,氮、铁、锌和硼的含量分别相差12.06、6.17、7.7和10.08倍,果实可滴定酸含量和固酸比分别相差2.33和2.16倍;果实品质与果实矿质元素含量的相关分析表明,果实氮含量与果实可溶性固形物含量、固酸比和着色面积呈极显著的负相关,果实中钙、铁含量与果实可溶性固形物含量和固酸比呈极显著的正相关;偏最小二乘回归的模型效应载荷和权重分析表明,果实氮和铁对果实综合品质性状影响较大,其次是果实中的磷、钾和钙;果实单果质量主要受果实中氮、磷、钾的影响,并与磷、钾含量有较大的正效应系数,而与氮含量有负的效应系数;变量投影重要性获得了果实可溶性固形物含量主要受果实中氮、钙和铁的影响,并与果实氮含量有最大的负效应系数,而与果实铁和钙有正效应系数。‘富士’苹果优质的果实矿质元素最佳质量分数为:氮0.25%、磷0.07%~0.12%、钾0.7%~1.2%、钙700 mg?kg-1、铁105~184 mg?kg-1、锌4.3~9.0 mg?kg-1、硼16.8~43.0 mg?kg-1。【结论】降低果实氮含量,提高果实铁、磷、钾和钙含量是提高‘富士’果实品质的重要技术措施。
张强李兴亮李民吉周贝贝孙健魏钦平
关键词:偏最小二乘回归
核桃基因组DNA甲基化修饰位点的MSAP分析被引量:3
2014年
以‘中宁强’核桃品种成熟叶片为材料,采用优化的MSAP反应体系,在全基因组水平对核DNA进行甲基化修饰位点分析,结果表明:选用20对引物组合,共扩增出1 060条清晰、重复性好的谱带,平均每对引物扩增出53条谱带,其中,甲基化位点241个,甲基化修饰比例为22.73%。对部分核桃DNA甲基化修饰位点进行回收测序,得到10条存在DNA甲基化修饰的DNA序列,BLASTn分析表明,核桃基因组中多种类型的DNA序列存在甲基化修饰现象。
周贝贝文明马庆国裴东
关键词:核桃MSAPDNA甲基化表观遗传学
核桃亲子鉴定方法的建立被引量:9
2011年
【目的】针对核桃育种中父本不清、亲本混淆问题,建立简便快捷高效的核桃亲本鉴定方法,也为其它林木和果树品种亲本鉴定提供参考。【方法】以‘鲁果2号’为鉴定材料,采用表型性状和SSR标记的方法对‘鲁果2号’及其可能的亲本进行亲子分析。【结果】表型性状方法统计的8对坚果性状在各品种间差异很小,不能鉴定出子代亲本;SSR分型检测出疑似亲本中只有‘上宋6号’和‘鲁香’与子代‘鲁果2号’的等位基因遗传符合孟德尔遗传规律,其累积RCP(亲子关系概率)为99.997%,‘slz-40’与已知亲本鉴定结果与实际相符。【结论】‘上宋6号’和‘鲁香’是‘鲁果2号’的亲本,SSR分型技术较好地鉴别出了‘鲁果2号’的亲本,为其它林木和果树品种寻找和鉴别亲本,提供有效手段。
周贝贝马庆国王滑王伟张美勇裴东
关键词:核桃表型性状SSR亲子鉴定
苹果矮化砧木抗旱、寒研究进展
2015年
干旱、低温是影响我国苹果生产的主要逆境因素,苹果矮化砧木直接影响着树体的生长发育、产量及果实品质的形成。苹果矮砧集约栽培已经成为世界栽培的发展方向,也是我国苹果产业的发展趋势。本文对苹果砧木抗旱、寒研究方法、试验材、相关指标测定和抗旱、寒性综合评价等方面进行了系统总结,并结合模式植物的抗性机理研究,对苹果矮化砧木抗旱、寒生理及分子机理研究进行了综述,为苹果矮化砧木的抗旱、寒筛选、评价和利用及新的矮化砧木培育提供理论依据和参考。
李兴亮张强张强周贝贝孙健魏钦平
关键词:抗旱苹果矮化砧栽培
不同砧木品种对核桃树体生长及光合特性的影响被引量:17
2017年
[目的]采用将同一品种嫁接到不同砧木的方法,经过调查测试和分析,揭示杂交种‘中宁奇’的砧木特性,进而阐明砧木在经济林育种和栽培中的地位和作用。[方法]以砧穗组合上宋-14/中宁奇和上宋-14/宁优为试材,测定分析不同砧穗组合的生长量和光合特性指标。[结果]嫁接亲和性调查显示:2种砧木对同一核桃品种的嫁接亲和性均较高,但差异不显著。树体生长特性调查表明:上宋-14/中宁奇树高、干径、冠幅、新梢长度、新梢粗度、新梢着生小叶数及分枝力等指标显著或极显著高于上宋-14/宁优,‘中宁奇’作砧木可显著增强核桃树体的生长势。光合特性分析结果显示:2种砧穗组合间光响应曲线特征参数存在显著差异,上宋-14/中宁奇最大净光合速率(21.93μmol·m^(-2)·s^(-1))、光饱和点(1 550μmol·m^(-2)·s^(-1))及表观量子效率(0.062 mol·mol^(-1))分别比上宋-14/宁优提高17.90%、11.51%和16.98%,而光补偿点(16.53μmol·m^(-2)·s^(-1))则显著降低35.33%。[结论]核桃杂交种‘中宁奇’与‘上宋-14’核桃品种有较强的亲和性,以‘中宁奇’作砧木可显著提高树体的生长量和光合能力,使嫁接品种对光强的利用范围变广,从而提高树体对光环境的适应性。
李莉周贝贝徐慧敏张俊佩裴东
关键词:光合特性光响应曲线
4种矮化自根砧对宫藤富士苹果抗盐性的影响
2024年
比较了盐胁迫对不同矮化自根砧宫藤富士苹果生长的影响,为土壤盐碱化地区苹果矮化自根砧木的选用与抗盐机理研究提供参考。以SH6、M9T337、G935、G114种矮化自根砧宫藤富士苹果2年生盆栽苗为试材,浇灌0.5%NaCl、1.0%NaCl溶液进行胁迫处理,以浇灌去离子水为对照,在持续处理60 d后调查盐害指数,测定生长、矿质元素含量等指标。结果表明:G11矮化自根砧宫藤富士植株盐害指数最高,其次为SH6矮化自根砧和M9T337矮化自根砧,G935矮化自根砧宫藤富士植株盐害指数最低,表现最为抗盐;盐胁迫下各矮化自根砧植株生长均受到显著抑制,生物量积累以G935矮化自根砧宫藤富士植株最高,G11矮化自根砧宫藤富士植株最低。在1.0%NaCl胁迫处理下,G11矮化自根砧富士叶片Na^(+)含量升高程度最大,为对照叶片的34.4倍;而G935矮化自根砧宫藤富士叶片Na^(+)含量升高程度最低,仅为对照叶片的2.4倍。4种矮化自根砧宫藤富士植株的抗盐性由强到弱依次为G935>M9T337>SH6>G11,抗盐矮化自根砧能够有效维持宫藤富士植株地上部低钠水平可能是其抗盐性形成的关键因素。
周佳李兴亮李民吉周贝贝杨雨璋张强魏钦平张军科
关键词:苹果盐胁迫
核桃砧木对树体影响的DNA甲基化调控机制研究
嫁接一方面保持了品种性状相对一致性,另一方面不同砧木也会明显改变树体的表型。砧木如何影响接穗表型性状,其分子机制有待深入研究。本研究将‘上宋-14’核桃品种分别嫁接到‘中宁奇’和‘宁优’砧木上,发现核桃嫁接苗树体在表型性...
周贝贝
关键词:核桃MSAP表型性状表观遗传学
Study and Application of Partial Least Squares Regression on Relationship between Soil Nutrient and Fruit Quality被引量:3
2016年
[Objective] The objective of the study was to elucidate the duplicate collinearity diagnosis method for soil nutrient and provide basis for establishment of multiple regression model of relationship between independent variable and depen- dent variable in fruit trees. [Method] An investigation on soil nutrients and 'Fuji' fruit quality was conducted at 153 commercial apple orchards in the production re- gions of 51 counties in China from 2011 to 2012. At first, the regression equation was used to do the duplicate collinearity diagnosis, and then the partial least squares regression was used to analyze the weights of different soil nutrients that affected the fruit quality and screen out main soil nutrients. [Result] The results showed the conditional number between soil nutrients was 61.31, and the correlation coefficient of soil pH and available calcium, iron was 0.82 and -0.824 respectively, which demonstrated that there was multicollinearity between various soil nutrient contents. The soil organic matter, total nitrogen, available potassium, available nitro- gen, available phosphorus, available zinc, available iron and available boron showed positive effect Ioadings on fruit quality, while the }oadings and weights of the effects of available calcium and pH on fruit quality were negative. The variable importance for projection method was adopted to get the orders of the effects of major soil nu- trients on fruit quality, which was in the order of soil available calcium, nitrogen, available iron, available phosphorous, organic matter and available boron. The solu- ble solid content of the fruit was affected by soil organic matter, available phospho- rus, available iron, available boron and soil pH. [Conclusion] This study provided an analysis method and theoretical basis for the study and analysis on the relationships between dependent variables and multi-objective optimization, as well as the effects of soil nutrients on fruit quality in fruit tree.
周贝贝张强孙健李兴亮魏钦平
关键词:APPLEPLS
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