[ Objective ] This study was to express and purify Arabidopsis thaliana heat shock factor HSF1. [ Method ] Using Escherichia coli M15 harboring HSF1 （pQE32/His6-HSF1, pREP4） as experimental materials, HSF1 was induced to express with isopropyl-β-D-galactoside （IPTG） ; then the expression product was purified using Ni-NTA-agarose affinity chromatography and analyzed by SDS-PAGE. [Result] HSF1 of Arabidopsis thaliana was successfully expressed and purified. [ Conclusion] This study provides materials for understanding the blinding site of HSF1 on Arabidopsis thaliana chromosome, further laying a good foundation for revealing the regulatory mechanism and physiological function of HSF1.
With Firmiana simplex （L.） W. F. Wight as an experiment material, the effects of different concentrations of GA3 （Gibberellin A3）, SA （Salicylic acid） and NAA （1-Naphthalene acetic acid） and stratification on the germination rate of seeds were studied firstly, and then the effects of GA3 and NAA on seedling growth were analyzed. The results showed that the germination rate could be improved by 0.075 g/L SA and stratification markedly. It was indicated by the experiment that the growth of the main root and main stem could be promoted by 0.050 g/L and 0.077 g/L NAA, respectively. However, the leaf length and leaf width decreased significantly in the GA3 and NAA treatments.
