The genetic fingerprints of Chinese wildrye ( Aneurolepidium chinensis (Trin.) Kitag) were constructed by Southern blot analysis of Ase Ⅰ_, Dra Ⅰ_, Eco RⅠ_, Hin dⅢ_, Nco Ⅰ_, Mob Ⅰ_, Rsa Ⅰ_, Pst Ⅰ_, Taq Ⅰ_ digested genomic DNA probed with synthesized oligonucleotide,(CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 .The difference between “Jisheng 4" and Chinese wildrye was shown by RFLP analysis of Dra Ⅰ_ or Rsa Ⅰ_ digested genomic DNAs probed with (GCTA) 4 .DNA fragments were obtained by PCR performed with genomic DNA of “Jisheng 4" as a template and (CT) 8,(GCTA) 4,(GGAT) 4 or (GACA) 4 as primer. Five size 2325,1455, 876,774 and 299 bp fragments were amplified when (GGAT) 4 was used, indicating satisfied results were obtained.
The fingerprints of 27 Leymus chinensis (Trin.) Tzvel accessions from different regions in China were generated and their genomic DNA variations were assayed via amplified fragment length polymorphism (AFLP). A total of 537 DNA fragments were amplified ranging from 75-530 bp using 8 AFLP primer combinations, among which 89 (16.6%) were monomorphic and 329 (61.3%) were polymorphic. The average number of DNA band produced by each primer combination was 66.13 and the total polymorphic rate was 78.84%. PICs (polymorphic information contents) scores for AFLP ranged from 0.0-0.5 and the mean PIC was 0.216. PIC scores were maximum (0.5) for 8.5% of the AFLP markers. All the results revealed that there was rich polymorphism in genomic DNA of L. chinensis . A DNA molecular dendrogram was established for 27 L. chinensis genotypes based on genetic variation and UPGMA cluster analysis of 537 AFLP markers amplified by 8 primer combination, suggesting that the genetic variation with high frequency showed close correlation with their ecotopes and geographical distributions. Twenty_seven L. chinensis genotypes could be divided into four groups. Among them the lower genetic relationship was shown. The reason of high genetic variation frequency in L. chinensis species and formation of varieties was discussed.
