目的:探讨电针丰隆穴对高脂血症大鼠血脂水平、肝脏组织的清道夫受体BI(scavenger receptor clas s B type I,SR-BI)、过氧化物酶体增殖物激活型受体γ(peroxi some proliferator-activated receptor-γPPARγ)的mRNA相对表达量及腹腔巨噬细胞炎症相关因子细胞间黏附因子-1(intercellular adhesion molecule-1,ICAM-1)、白介素-1β(intefleukin-1β,IL-1β)、IL-10含量的影响及其相关作用机制.方法:将SD大鼠随机分为空白对照组、模型对照组、电针1组、电针2组这4组,每组10只.治疗结束后检测各组血脂含量,肝脏组织中SR-BI、PPARγ的mRNA相对表达量.最后分离出大鼠腹腔中的巨噬细胞,应用流式细胞仪检测炎症相关因子ICAM-1、IL-1β、IL-10的含量.结果:与空白对照组比较,模型对照组TG.变化并不明显(p>0.05),而总胆固醇(total chole sterol,TC)、低密度脂蛋白-胆固醇(loW density lipoprotein cholesterol,LDL-C)明显上升(P<0.01),高密度脂蛋白-胆固醇(high density lipoprotein cholesterol,HDL-C).明显下降(P<0.01);与模型对照组比较,电.针l组及电针2组的TG和HDL-C变化并不明显(P>0.05),大鼠血清TC、LDL-C均有显著下降(P<0.01);与电针1组比较,电针2组TC、LDL-C均有降低(P<0.01,P<0.05).与空白对照组比较,除了电针2组外,其余两组SR-BI、PPAR7均有显著降低(P<0.05,P<0.01);与模型对照组比较,电针1组和电针2组的SR-BI、PPARγ均有显著升高(P<0.05,P<0.01),其中电针2组更为显著(P<0.01).与空白对照组比较,除电针2组外,模型对照组和电针1组的炎症因子ICAM-1、IL-1β均有显著性升高(P<0.05,P<0.01),而模型对照组的抗炎因子IL-10有显著下降(P<0.01);与模型对照组比较,电针1组和电针2组的炎症因子ICAM-1、IL-1β均有非常显著性减少(P<0.01),而抗炎因子IL-10的含量有非常显著性升高(p<0.01).结论:通过电针丰隆穴治疗能降低高脂血症模型大鼠的TC、LDL-C水平,升高HDL-C水平,增加肝脏SR-BI、PPARγmRNA相对表�
Objective To explore the mechanism of electroacupuncture（EA） at ＂Fēnglóng＂（丰隆 ST 40） for hyperlipemia（HLP） in rats.Methods Forty health SD rats were randomly divided into a normal control group（group A）,a high fat forage group（group B） and a high fat forage ＋ treatment group（group C）,a high fat forage ＋ normal forage group（group D） and a high fat forage ＋ normal forage ＋ treatment group（group E）,eight rats in each group.EA was applied at ＂Fēnglóng＂（丰隆 ST 40） of the rats in group C and group E,once daily.After treatment of 30 days,plasma lipid levels in the rats,including total cholesterol（TC）,triglyceride（TG）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C） were detected.Real-time quantitative polymerase chain reaction（RT-PCR） and Western Blotting were applied to detect the gene expression changes of ATP-binding cassette transporter A1（ABCA1）,peroxisome proliferator-activated receptor alpha（PPARα）,liver X receptor alpha（LXR-α） and retinoid X receptor alpha（RXR-α） in liver tissue of rats.Results Compared with group A,contents of TC,LDL-C were significantly elevated in group B and group D（all P〈0.01）;compared with group B,above indices were significantly decreased in group D（all P〈0.01）.After the treatment of EA at ＂Fēnglóng＂（丰隆 ST 40）,contents of TC,LDL-C were significantly decreased（all P〈0.01）,and contents of TG,HDL-C did not change significantly（all P〉0.05）.Compared with group A,mRNA and protein contents of ABCA1,PPARα,LXR-α and RXR-α were decreased significantly in group B and group D（all P〈0.01）.But compared with group B,the above indices were decreased in group D.After the treatment of EA,mRNA and protein contents of ABCA1,PPARα,RXR-α and LXR-α significantly increased（all P〈0.05）.Conclusion EA at ＂Fēnglóng＂（丰隆 ST 40） can decrease contents of blood TC,LDL-C in rats of hyperlipemia and up-regulate the g
