The objective of this research was to develop gelatin microspheres（GMSs） and study their properties for embolization.The GMSs were prepared by emulsion chemical crosslinking method.The morphology and particle size of GMSs were observed under an optical microscope.The ratio of water absorption and GMSs swelling ratio were measured.The elasticity of GMSs was studied by TMS-Pro food texture analyzer.The deliverability of GMSs through catheter was investigated with a new device designed in this study.Finally,the acetone residue was determined by headspace capillary gas chromatography.The dried GMSs were elliptic with corrugated surface and the wet GMSs were round with smooth surface.The average diameter of dried GMSs was 430.5 μm with a range of 100-1000 μm,and that of wet GMSs was 601.2 μm with a range of 150-1425 μm.The maximum ratio of water absorption was 590.7% and the average swelling ratio was 103.0%.The elasticity of GMSs was proven to be appropriate for embolization.Three subgroups of wet GMSs（100-450 μm,450-700 μm and 700-940 μm） were delivered through catheter with acceptable pressure.The content of residual acetone in GMSs was less than the minimum limit in the Chinese Pharmacopoeia 2010.Therefore,we concluded that the GMSs were suitable for embolization according to the applicable properties in vitro.And the methods for assessing the properties of GMSs in this study were going to be useful in the evaluation of embolic microspheres.
目的:研究借助磁共振成像(magnetic resonance imaging,MRI)可定位的Eudragit-E液体栓塞剂(MR-E)的制备方法,并对其体外和体内的性质进行研究。方法:采用化学共沉淀法制备聚乙二醇修饰的超顺磁性氧化铁(polyethylene glycol-modified superparamagnetic iron oxides,PEG-SPIO),将PEG-SPIO和Eudragit-E液体栓塞剂混合均匀制得MR-E。通过体外MRI实验考察MR-E的成像性质,并筛选PEG-SPIO的浓度。通过经微导管推注实验和体外固化实验考察MR-E的推注性能和固化性质。以家兔肾动脉为栓塞模型,考察MR-E的体内栓塞效果和磁共振成像性质。结果:MR-E中PEG-SPIO的浓度确定为2 g/L,MR-E具有良好的推注性能和固化性质,0.2 mL MR-E注入家兔肾动脉后,可以有效地栓塞肾动脉远端血管,并且通过MRI可以示踪MR-E的组织分布情况。结论:MR-E有望成为一种新型的借助MRI可定位的液体栓塞剂。
