The Erns gene was amplified by RT-nPCR from C-strain of Classical swine fever virus(CSFV).Then the Erns gene was cloned into pGEX6P-1 vector.The expression products were analysed by SDS-PAGE and Western blotting methods.The inclusion bodies were recovered from bacterial lysate by centrifugation and washed with buffer,and then dissolved in denaturing agents.The protein was refolded by dilution dialysis.Refold protein reacted strongly with the positive serum of CSFV,which indicated that the protein has immunogenic.
