[Objective] The paper was to establish embryonic stem cell system of goats. [Method] Numerous primordial germ cell colonies were derived from gonadal ridge and the surrounding tissues in 20 millimeter fetuses of down producing goat. Primordial germ cells and goats embryonic fibroblasts obtained from conceptus of equivaient gestational age were co-cultured. [Result] The colonies showed some characteristics of embryonic stem cells, such as the morphology of nest-like, they continued to be AKP positive and the ability to be continuously passed [Conclusion] These cells were pluripotent and ES-like cells.
ISEC (International Scholarly Exchange Curriculum)项目是国际本科互认课程,该项目旨在通过教育的国际化来深化高等教育,将崭新的教育理念融入中国本科教育。论文对ISEC项目下微生物学教学进行了分析和探讨,描述了传统教学模式微生物学面临的困境和ISEC项目下微生物学面临的机遇和挑战,并对ISEC项目理念下微生物学教学提出了改进建议,这些探讨对于ISEC教育理念下微生物学的课程改革具有一定积极意义。
