This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ultrasonic extrac-tion of pachyman. Firstly, the ultrasonic power, extraction time and solid-to-liquid ra-tio were investigated by ultrasonic extraction method. Then, on this basis, a respon-sible surface experiment was designed, to select the optimal process conditions ac-cording to the index of pachyman yield. The optimized ultrasonic water extraction conditions were as fol ow： ultrasonic power of 100 W, extraction time of 30 min, and solid-to-liquid ratio at 1：40. The Oxford cup method was used to study the an-timicrobial activity of pachyman. lt was found that pachyman could significantly in-hibit Bacilus subtilis_and Staphylococcus aureus.
In this study, with Jiuzihe poria as the main raw material, the effect of alkali concentration, extraction time and solid-liquid ratio on the extraction rate of alkali-soluble polysaccharide was investigated, and the processing technology was optimized with orthogonal experiment. The results showed that the optimum extraction process for alkali-soluble polysacchadde from poria was as follows： solid-liquid ratio of 1：50, alkali concentration of 0.5 mol/L, and extraction time of 8 h.
