搜索到33篇“ CTX-M-15“的相关文章
一株同时携带bla_(CTX-M-14)和bla_(CTX-M-15)基因的多重耐药宋内志贺菌耐药基因及质粒分析
2023年
目的对1株同时耐头孢菌素和阿奇霉素的多重耐药宋内志贺菌进行全基因组测序分析,明确其携带的耐药基因和质粒特征。方法通过对2011年上海市的60株宋内志贺菌进行抗菌药物敏感性分析,筛选出1株同时耐头孢菌素和阿奇霉素的菌株Sh11sh529,使用二代和纳米孔测序技术获得其基因组序列,分析基因组的耐药基因、质粒、可移动遗传元件等。结果Sh11sh529基因组中检测到了11个耐药基因以及gyrA基因喹诺酮耐药决定区(QRDR)的突变位点gyrA-S83L,耐药基因包括2种CTX-M型超广谱β内酰胺酶(ESBL)基因bla_(CTX-M-14)和bla_(CTX-M-15),其中bla_(CTX-M-14)、mphA、aac(3)-IId、dfrA17、aadA5、sul1和qacEdelta基因由1个IncB/O/K/Z型质粒pSh11sh529-2携带,bla_(CTX-M-14)上游有1个ISEcp1插入子,mphA上游有1个IS26插入子。而bla_(CTX-M-15)由IncFII型质粒pSh11sh529-3携带,在其上游有1个ISEcp1插入子。该菌株表现出多重耐药性,对头孢菌素类、大环内酯类、氨基糖苷类、磺胺类、青霉素类等5类抗菌药物中的9种耐药。结论应当加强对多重耐药菌株以及同时携带多个类型ESBL菌株的监测和研究,以制定策略减少其流行和传播。
刘康康李春晓
关键词:宋内志贺菌
产NDM--1&CTX--M--15肠杆菌科细菌的分子特征及小鼠感染非靶向代谢组学研究
本文从两部分进行了阐述:  第一部分,对实验室筛选出的3株同时携带blaNDM-1和blaCTX-M-15的碳青霉烯类耐药肠杆菌科菌株进行耐药表型验证、耐药基因PCR检测、细菌多位点序列分型(MLST)、耐药基因质粒定位...
张佳璇
关键词:肠杆菌科细菌分子特征代谢组学生物标志物
CTX-M-15型大肠埃希菌的分子分布特征及质粒传播规律研究被引量:4
2017年
目的探讨CTX-M-15型大肠埃希菌的分子分布特征及质粒传播规律。方法 232株CTX-M-15型大肠埃希菌鸟枪法测序序列从GenBank数据库获取,利用CGE在线工具获得菌株的MLST分型、耐药基因及质粒类型信息,应用eBURST软件绘制MLST分型结果聚类图。结果 232株CTX-M-15型大肠埃希菌共检测出37个MLST型别,其中以ST131菌株检出率较高,为35.34%(82/232)。菌株中耐药基因数目为1~22个不等,出现耐药基因数目较多菌株的ST型别为ST131和ST617,其中blaOXA-1、aac(6')Ib-cr、aac(3)-II、aadA、mph(A)等7种耐药基因在ST131菌株和非ST131菌株中的分布差异有统计学意义(P<0.05或P<0.01),且ST131菌株检出率高于非ST131菌株(P<0.05或P<0.01)。菌株中检测到blaOXA-48、blaKPC-2、blaNDM-1、blaCMY、blaVIM等其他类型的β-内酰胺类耐药基因,主要的β-内酰胺基因组合形式是blaTEM-1+blaCTX-M-15,而在ST131中主要的组合形式是blaOXA-1+blaCTX-M-15(32/82)。检测到的不相容性质粒的类型有9种,其中IncF型质粒的检出率较高,为95.26%(221/232),该质粒在ST131菌株中的检出率为98.78%(81/82)。菌株中IncF型复制子类型出现频率较高的是IncFIA+IncFIB+IncFII(83/232),在ST131菌株中上述复制子中检出率较高的是IncFIA+IncFII,为51.22%(42/82)。IncF型质粒经pMLST分型可获得59种型别,F36:A4:B1、F31:A4:B1、F48:A1:B49型质粒主要存在于ST131菌株和CC10克隆复合体中,而ST131菌株中主要的质粒类型是F2:A1:B-(38/82)。结论 CTX-M-15型耐药基因主要存在于ST131型别的大肠埃希菌株中,ST131菌株比其他菌株具有更强的适应能力,同种型别的质粒可以在亲缘关系相近的菌株之间传播。
徐亚珂龙金照段广才梁文娟梁文娟陈帅印郗园林
关键词:大肠埃希菌CTX-M-15MLST
多药耐药大肠埃希菌携带CTX-M-14与CTX-M-15酶基因的研究被引量:1
2016年
目的了解多药耐药大肠埃希菌携带质粒介导的CTX-M-14与CTX-M-15酶基因分布及其对常见抗菌药物的耐药性,为临床合理选择抗菌药物提供依据。方法收集2007年7月-2008年7月感染患者送检标本中分离出的大肠埃希菌71株,采用纸片扩散法(K-B法)对其中多耐30株、双耐19株及单耐16株大肠埃希菌进行药物敏感试验,提取质粒DNA,以此为模板采用聚合酶链反应(PCR)法对CTX-M-14与CTX-M-15酶基因进行扩增,PCR产物送上海生工进行基因测序。结果 71株大肠埃希菌耐药模式为多耐30株、双耐19株、单耐16株、全敏6株,分别占42.2%、26.8、22.5%、8.5%;56株多药耐药大肠埃希菌对亚胺培南、阿米卡星的耐药率较低,<15.0%,对头孢噻肟、氨曲南、庆大霉素等的耐药率均>50.0%;56株多药耐药大肠埃希菌中,44株检出CTXM-14酶基因,28株检出CTX-M-15酶基因,检出率分别为78.6%和50.0%;携带CTX-M-14与CTX-M-15酶基因的多药耐药大肠埃希菌对头孢噻肟、头孢他啶的耐药率分别81.8%、52.2%和96.4%、60.7%,两者相比较,差异有统计学意义(P<0.01)。结论多药耐药大肠埃希菌中,含CTX-M-14与CTX-M-15酶基因菌株是常见的,携带CTX-M-14与CTX-M-15酶基因的多药耐药大肠埃希菌对头孢噻肟耐药率高于头孢他啶。
罗瑜黄勇陈枫林雁黄永茂钟利向成玉陈庄
关键词:大肠埃希菌多药耐药
CTX-M-14和CTX-M-15大肠埃希菌毒力基因分布差异被引量:4
2016年
目的分析尿培养产CTX-M-14和产CTX-M-15大肠埃希菌毒力基因分布的差异。方法收集南京鼓楼医院2012年临床尿培养分离大肠埃希菌162株,双环协同试验检测超广谱β内酰胺酶(ESBLs);采用PCR和DNA测序对CTX-M。编码基因及毒力基因iutA、ompT、fyuA、fdeC、fimH、traT、cvaC,pap、kpsMr、PAls、usp、aer、hlyA、cnf和chuA进行分析;用脉冲场凝胶电泳(PFGE)分析产CTX-M-14和产CTX-M-15大肠埃希菌遗传相关性;将细菌分为产CTX-M-14和产CTX-M-15大肠埃希菌2组,统计学分析毒力基因在这2组之间的分布差异。结果162株大肠埃希菌中,126株产ESBLs,91株产CTX-M酶,其中blaCTX-M-14和blaCTX-M-15编码基因分别检出49株和36株。PFGE分析显示,产CTX-M酶大肠埃希菌具有遗传多样性。毒力基因iutA、fyuA、fimH、traT及chuA在2组中的检出率均较高(〉65%);pap、kpsMV、ompT在2组中的检出率约为20%-60%;cvaC和PAls在2组中的检出率较低(〈20%);毒力基因fedC在产CTX-M-14大肠埃希菌组中分布高于产CTX-M-15大肠埃希菌组(P=0.017);未检出aeF、hlyA和cnf毒力基因。结论在尿培养大肠埃希菌中,产CTX-M-14菌株fedc毒力基因分布较产CTX-M-15菌株更高。
许元元沈瀚张之烽宁明哲周万青曹小利
关键词:毒力大肠埃希菌
耐多药大肠埃希菌携带CTX-M-14,CTX-M-15酶基因的检查研究
目的:了解本地区耐多药大肠埃希菌携带质粒介导的CTX-M-14和CTX-M-15酶基因分布情况及对常见抗菌药物的耐药情况,并对溴化乙啶(EB)消除耐药质粒的影响进行探讨。  方法:1.CTX-M-14和CTX-M-15酶...
黄勇
关键词:大肠埃希菌
文献传递
恶臭假单胞菌CTX-M-15型β内酰胺酶基因的检测
2014年
产超广谱β内酰胺酶(extended spectrum beta—lactamases,ESBLs)是肠杆菌科细菌对头孢菌素类抗菌药物耐药的主要机制[1]。目前已经有超过10类的ESBLs被发现,主要包括CTXM、SHV、TEM、PER、VEB及OXA等[4],这些ESBLs主要分布于肠杆菌科细菌及非发酵菌中的鲍曼不动杆菌和铜绿假单胞菌中,在其他菌种中较少见[2]。恶臭假单胞菌常从腐败的鱼、鸭及土壤中检出,也是人类咽部的正常菌群之一,可导致人类泌尿道感染、皮肤感染等[3]。
孙杰孙景勇
关键词:恶臭假单胞菌产超广谱Β内酰胺酶肠杆菌科细菌ESBLS抗菌药物耐药
CTX-M-15基因在大肠埃希菌和肺炎克雷伯菌头孢他啶耐药株中的检测被引量:3
2012年
[目的]了解CTX-M-15基因与大肠埃希菌和肺炎克雷伯菌对头孢他啶耐药的关系。[方法]筛选ESBLs阳性且对头孢他啶耐药的菌株为实验组,对头孢他啶敏感而对头孢曲松耐药的菌株为对照组。多重PCR方法检测CTX-M-14,CTX-M-15基因。[结果]实验组CTX-M-15基因检出率高达75.9%,单纯CTX-M-15基因为40.6%,同时存在CTX-M15CTX-M-14基因为35.2%。而对照组检出率分别为:17.7%、5.9%、11.8%。[结论]CTX-M-15基因是大肠埃希菌和肺炎克雷伯菌对头孢他啶耐药的主要机制,建议临床合理使用抗生素,避免引起流行。
李瑞华聂大平何丽敏
关键词:CTX-M-15大肠埃希菌肺炎克雷伯菌
Prevalence of plasmid mediated bla_(TEM-1) and bla_(CTX-M-15) type extended spectrum beta-lactamases in patients with sepsis被引量:2
2012年
Objective:To characterize the bacterial pathogens in patients having gram negative septicaemia. Further,to evaluate the antimicrobial resistance and underlying molecular mechanisms in these strains.Methods:A total number of 70 cases of gram negative sepsis were included in this prospective,open labeled,observational study.Standard methods for isolation and identification of bacteria were used.Antimicrobial susceptibility and ESBL testing was performed by the standard disc diffusion method.PCR amplification was performed to identify blaCTX-M,blaSHVand blaTEM type ESBLs.Conjugation experiments were performed to show resistant marker transfer. Results:The most prevalent isolates Escherichia coli(E.coli) 58.6%,Klebsiella Spp.32.9%and Pseudomonas 8.6%,were resistant to most of the antimicrobials including cefazolin,ceftriaxone, cefuroxime,ampicillin and co-trimoxazole but sensitive to imipenem and meropenem.ESBL and MBL production was seen 7.3%and 12.2%of E.coli isolates respectively.Three isoaltes were found to have blaCTX-M-15 and two of them also showed blaTEM-1 type enxyme.Whereas, none of them showed blaSHV.Conjugation experiments using J-53 cells confirmed these resistant markers as plasmid mediated.Conclusions:This work highlights the molecular epidemiology of escalating antimicrobial resistance and likely switch over of blaCTX-M-15 type extended spectrum beta-lactamases by blaTEM type ESBLs in India.Further,the antimicrobial resistance by horizontal gene transfer was predominant among Enterobacteraceae in the community setting.
Shahzad F HaqueSaeedut-Z AliMohammed TPAsad U Khan
关键词:SEPSISESBL
Increasing secondary bacterial infections with Enterobacteriaceae harboring bla_(CTX-M-15) and bla_(CMY-6) in patients with bronchogenic carcinoma:an emerging point of concern被引量:1
2011年
Objective:To look for secondary bacterial infections in bronchogenic carcinoma(BCA) with resistant organisms harboring bla genes considering the paucity of relevant studies.Methods: A total of 137 confirmed cases of BCA and 34 healthy volunteers were studied for the occurrence and prevalence of blaCTX-M and and blaAmpC harboring-enterobacteriaceae.A subset of these patients(n=69) was previously reported for the secondary infection with the Aspergillus species. Bronchoalveolar lavages(BAL) were subjected for bacterial and fungal cultures and the bacterial isolates were screened by multiplex PCRs for the presence of blaCTX-M and blaAmpC.The isolates were also screened for the association of insertion sequence(IS26) by PCR and characterized by RAPD for any clonal relatedness.Results:A total of 143 bacterial isolates were obtained from 137 BAL specimens of BC,patients.The Enterobacteriaceae-isolates were multidrug-resistant showing concomitant resistance to fluoroquinolones and aminoglycosides.Both blaCTX-M and blaAmpC of CIT family were detected in 77.4%and 27.4%isolates,respectively.Sequencing revealed the presence of blaCTX-M-15 and blaCMY-6.Twenty one percent of the isolates were simultaneously harboring blaampC and blaCTX-M-15.IS26 PCR and RAPD typing revealed the presence of diverse bacterial population but no predominant clone was identified.The present study also suggests strong association of aspergillosis with lung cancer and further strengthens the potential use of non-validated serological tests suggested earlier.Conclusions:We emphasize that all patients of bronchogenic carcinoma should also be screened for secondary bacterial infections,along with secondary fungal infections,so as to introduce early and specific antimicrobial therapy and to prevent unwanted deaths.
Mohammed ShahidAbida MalikRakesh Bhargava
关键词:SECONDARYCTX-MENTEROBACTERIACEAE