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Evaluation of the hepatoprotective and antioxidant effects of Tegillarca granosa flesh body extract against potassium bromide toxicity via targeting the histomorphometry,chromosomal and expressions of TGF-β1,VEGF and COX-2 genes in rats被引量:1
2022年
The hepatotoxic effect of potassium bromide(KBr)on rat liver tissues were determined,as well as the potential protective effect of Tegillaraca granosa(T.granosa)flesh body extract.Twenty adult male albino rats were equally distributed into four groups;Group(I)treated with physiological saline(control group),Group(II)was orally gavaged by 200 mg/kg of T.granosa body extract day after day,Group(III)was intoxicated by KBr(150 mg/kg bwt day after day orally)and finally,Group(IV)was given a combination of T.granosa flesh body extract plus KBr with similar doses in the second and third groups.At the end of one month,blood,liver tissue and bone marrow samples were collected to be used for the required laboratory examinations.In response to KBr toxicity,there was a significant increase in serum antioxidant biomarkers,which was accompanied by a significant change in hepatocyte ultrastructure and a significant change in carbohydrate and protein levels within the liver organ.In addition,KBr intoxication resulted in a substantial increase in the incidence of chromosomal aberrations such as holes,splits,deletions,fragments,ploidy,and ring chromosomes,as well as significant upregulation of TGF-1,VEGF,and COX-2 gene expression.The hepatotoxic effect of KBr was counteracted by treatment with T.granosa flesh body extract.T.granosa flesh body extract has a curative antioxidant and numerous protective effects against KBr hepatotoxicity.
ALI H.ABU ALMAATYBASMA M.HENDAMFAYEZ ALTHOBAITIEMAN FAYADYASMIN M.ABD EL-AZIZ
关键词:HISTOMORPHOMETRY
不同骨密度骨质疏松性椎体压缩骨折骨组织形态与骨代谢标志物分析被引量:11
2022年
目的观察不同骨密度骨质疏松椎体压缩骨折(osteoporotic vertebral compression fracture,OVCF)骨组织形态学特征及骨代谢标志物变化规律。方法将136例OVCF患者按不同骨密度(T值)分为3组:I组,-3.5
张芸张业峰王磊李志超王文波薛景才
关键词:骨质疏松椎体压缩骨折骨密度骨组织形态学骨代谢标志物
Histomorphometry of the Testes, Epididymides, and Spermatozoa of Four Indigenous Breeds of Bulls in Ibadan, Nigeria
2021年
Forty testicles were used to carry out histomorphometry study on the testes, epididymides and spermatozoa of four indigenous breeds of bull found in Ibadan (Red Bororo (RB), Sokoto Gudali (SG), White Fulani (WF) and Dhali (DL)). The testicles were harvested immediately after the slaughter at the Bodija abattoir and transported to the laboratory in an insulated flask containing warm water at 37°C within 30 minutes. In the laboratory, the samples were dissected and histological sections of the right testis, right and left caudal epididymides taken from two bulls in each group. The tissues were fixed in Bouin’s fluid. They were processed in an automatic tissue processor, embedded in paraffin wax using the embedding system (Leica EG 1160) and sectioned with microtome at 4 microns. The sections were then stained by Haematoxylin and Eosin method and mounted and examined under a microscope. There was no significant difference between breed and seminiferous tubular diameter (STD) and testicular germinal height (TGEH). The mean STD ranged from 223.00 ± 28.35 to 316.00 ± 37.70 μm, while the TGEH ranged from 81.60 ± 10.05 to 89.80 ± 4.83 μm. The mean epididymal tubular diameters (ETD) and epididymal lumen diameters (ELD) had the highest value of 378.00 ± 10.95 and 298.20 ± 26.0489 μm in DL and lowest of 373.80 ± 37.70 and 278.10 ± 27.71 μm in RB, respectively. The mean epididymal germinal epithelia heights (EGEH) were highest (61.20 ± 5.70 μm) in WF and lowest (39.80 ± 0.86 μm) in RB. The mean sperm head length (SHL), sperm midpiece length (SMPL), sperm tail length (STL) and sperm total length (TL) had the highest values of 5.90 ± 0.10, 8.80 ± 0.25, 24.20 ± 2.59 and 38.90 ± 2.94 μm, respectively in SG. SHL and SMPL were lowest in DL bulls (5.00 ± 0.00 and 6.30 ± 0.20 μm), while STL and TL
Ayodeji Ayotunde OniMatthew Olugbenga OyeyemiJoseph Atawalna
关键词:HISTOMORPHOMETRYTESTESEPIDIDYMISSPERMATOZOA
Vitamin D3 supplementation influences ovarian histomorphometry and follicular development in prepubertal albino rats被引量:1
2020年
Objective:To evaluate the development of ovarian follicles in female albino rats following vitamin D3 supplementation.Methods:Eighteen prepubertal female albino rats,aged 3-4 weeks,weighing(70.25±9.16)g,were assigned to three groups(n=6 in each group).Group A was treated with 5.00 mL/kg of distilled water and served as the control group,group B was treated with 0.025 mg/kg of vitamin D3 dissolved in distilled water,and group C was treated with 0.125 mg/kg of vitamin D3 dissolved in distilled water.All treatments were administered orally,twice weekly for 28 days.Blood and ovaries were harvested under anaesthesia.Serum vitamin D3 levels were determined by using spectrophotometric method.Ovaries were processed for histology and every10th hematoxylin and eosin stained-section was selected for histomorphometry.The number of follicles at each developmental stage was estimated.Results:Both 0.025 mg/kg and 0.125 mg/kg of vitamin D3 significantly increased serum concentrations of vitamin D3 and calcium(P<0.05),but did not alter inorganic phosphorus concentration(P>0.05).The control group had fewer growing follicles(primary,secondary and antral follicles)and more non-growing follicles(primordial and atretic follicles)when compared with the vitamin D3-supplemented groups(P<0.05).Vitamin D3 at 0.025 mg/kg significantly increased antral follicles and corpora lutea counts(P<0.05).Vitamin D3 at 0.125 mg/kg significantly increased total,primordial and atretic follicles counts(P<0.05),but significantly decreased primary,secondary,antral follicles count,ovarian weight,relative ovarian weight,and ovarian surface area when compared with the control group and rats treated with 0.025 mg/kg of vitamin D3(P<0.05).Conclusions:Vitamin D3 supplementation at 0.025 mg/kg can enhance optimal ovarian follicle recruitment and development in female rats.
Edmund Chidiebere MbegbuIkechukwu Reginald Obidike
关键词:FOLLICULOGENESISHISTOMORPHOMETRYOVARY
A first comparison of bone histomorphometry in extant domestic horses(Equus caballus)and a Pleistocene Indian wild horse(Equus namadicus)被引量:1
2020年
The microstructural features of the tissue of long bones subjected to different biomechanical stresses could be a helpful tool for a better understanding of locomotor behavior in extant and extinct mammals,including equids.However,few researches have attempted to describe the bone tissue of extinct horses.In our study,we analyze and compare the histomorphometric features of the bone tissue in extant modern horses,Equus caballus,and Equus namadicus,a Pleistocene Indian extinct wild horse.The number,position,and size of the osteons and Haversian canals of the bone tissue,classifiable as dense Haversian tissue,were considered for the comparison.The results obtained highlight some differences between the analyzed species,E.caballus having fewer and bigger osteons than E.namadicus.The microstructural differences may depend on the different lifestyles and environmental conditions characterizing the two species.The results obtained suggest that comparing the biomechanical properties of extinct and modern horse species may provide indirect information on their paleoenvironment.
Marco ZEDDAVijay SATHEPrateek CHAKRABORTYMaria Rita PALOMBOVittorio FARINA
关键词:HISTOMORPHOMETRYHORSE
蓝刺头对绝经后骨质疏松症大鼠骨组织计量学及BMP-2、ADRβ-2R表达的影响
2020年
目的观察蓝刺头对绝经后骨质疏松(PMOP)大鼠骨组织计量学及骨形态发生蛋白因子-2(BMP-2)和骨β2肾上腺素能受体(ADRβ-2R)表达的影响。方法将84只雌性Wistar大鼠随机分为模型组、假手术组、强骨胶囊组(69.2 mg·kg^(-1))、己烯雌酚组(0.046 mg·kg^(-1))及蓝刺头高、中、低剂量组(550、275、137.5 mg·kg^(-1)),每组12只。以摘除卵巢法建立PMOP大鼠模型。造模结束后,大鼠按上述剂量灌胃给药,每日1次,连续90 d。采用显微CT法检测骨组织计量学指标,即大鼠股骨干骺端骨密度(BMD)、骨小梁体积分数(BV/TV);采用免疫组织化学法检测股骨头BMP-2及ADRβ-2的表达。结果与假手术组比较,模型组大鼠的BMD、BV/TV以及BMP-2的表达水平均明显下降(P <0.05,P <0.01),而ADRβ-2R的表达水平显著升高(P <0.01)。与模型组比较,蓝刺头各剂量组的BMD、BV/TV水平明显提高(P <0.01),蓝刺头中剂量组的BMP-2表达水平明显上调(P <0.05),而ADRβ-2R的表达水平明显下调(P <0.05)。结论蓝刺头能改善PMOP大鼠的骨强度,增加骨量,促进骨形成、抑制骨吸收,其作用机制可能与上调BMP-2表达,下调ADRβ-2R表达有关。
刘晋赵军
关键词:绝经后骨质疏松骨组织计量学骨形态发生蛋白-2
福美加对去势骨质疏松大鼠长骨干骺端骨组织计量学和生物力学的影响被引量:4
2020年
目的探讨福美加(阿仑膦酸钠维D3)对去势骨质疏松大鼠长骨干骺端骨组织计量学和生物力学的影响。方法30只3月龄雌性SD大鼠随机分为假手术组(Sham)、去卵巢组(OVX)和福美加组(ALN)。福美加治疗12周后,对所有大鼠进行骨密度测定,收集大鼠股骨和胫骨,用于骨组织计量学和生物力学指标的测定。结果ALN组股骨和L5椎体骨密度、胫骨骨质量参数(BV/TV、Tb.N、Tb.Th)明显大于OVX组(P<0.05)。Sham和ALN组股骨干及远端干骺端的最大载荷、破坏载荷和刚度明显高于OVX组(P<0.05)。OVX组与ALN组股骨干骺端弯曲试验最大载荷、破坏载荷的均值差异显著高于股骨干弯曲试验(P<0.05)。结论福美加可显著提高去势骨质疏松大鼠的骨密度、骨组织计量学参数和生物力学性能,且作用于长骨干骺端的效果大于长骨骨干。
袁伟权黎艺强陈志鹏林玮赵胜利莫小毅陈柏龄
关键词:骨质疏松干骺端骨组织计量学生物力学
蒙药蓝刺头抗绝经后骨质疏松症骨微观形态学计量研究被引量:8
2020年
目的通过研究证实蒙药蓝刺头防治绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)的作用。基于蒙医药"补暖补精-清镇赫依-固骨质壮骨"经典理论,通过防治PMOP提供实验依据,揭示此理论、治法效应,为蒙医药现代化提供思路。方法分组:6月龄SD系SPF级健康雌性未孕大鼠84只,体重(250±20) g,随机分为假手术组(Sham)、去卵巢骨质疏松模型组(OVX)、蒙药蓝刺头高剂量组(Echinops-H)、中剂量组(Echinops-M)、低剂量组(Echinops-L)、中药对照组淫羊藿组(HEP)、西药对照组辅酶Q10组(Co-Q10) 7组。造模:采用双侧卵巢切除法(去势)制备绝经后骨质疏松模型,阴道上皮角化实验、骨密度检测、子宫病理学检查验证造模成功。给药:术后90 d,模型组、假手术组给予生理盐水灌胃,其余各组予相应药物灌胃。标本采集:末次灌胃后行标本采集。处死大鼠剔除右侧完整股骨并标记,4%多聚甲醛溶液固定保存。指标检测:右侧股骨采用骨密度仪测定离体骨骨密度(bone mineral density,BMD)(股骨近端BMD、股骨总BMD)检测,行Micro-CT骨微结构形态计量学测定。结果去卵巢术后各组大鼠BMD下降明显,表现为松质骨、皮质骨骨量相继减少,皮质骨变薄,BMD下降;去卵巢术后各组大鼠骨组织体积、骨小梁体积分数、骨小梁厚度、骨小梁宽度、骨小梁数目、骨小梁模块因素下降明显,骨小梁分离度升高明显;适当剂量蒙药蓝刺头可以明显提高大鼠骨组织体积、骨小梁体积分数、骨小梁厚度、骨小梁宽度、骨小梁数目、骨小梁模块因素,明显降低骨小梁分离度;其具明显抗PMOP作用。结论蒙药蓝刺头对PMOP模型大鼠具显著抗骨质疏松作用;蒙药蓝刺头能显著促进模型大鼠骨组织成骨分化、骨形成;降低PMOP模型大鼠骨代谢高转换指标,抑制骨吸收。
赵军董重阳师建平刘晋党赢常虹海日李婧
关键词:绝经后骨质疏松骨组织形态学
氟化钠染毒致大鼠骨组织形态计量学及微小RNA-23a表达的变化被引量:4
2019年
[目的]探讨氟化钠染毒致大鼠骨组织形态计量学变化及对微小RNA(miR NA)-23a表达的影响。[方法 ]将96只SD大鼠按体重随机分为对照组(0 mg/L)、低氟组(50 mg/L)、中氟组(150 mg/L)、高氟组(250 mg/L)。采取饮水染毒方式,染氟至2、4、6个月时分别处死每组8只SD大鼠,收集大鼠左下肢及牙齿,采用灰化-氟离子电极法测定大鼠骨氟、牙氟含量;取大鼠股骨干骺端固定后脱钙,进行苏木精-伊红染色,光镜观察骨组织骨小梁、骺板、成骨细胞的形态学改变;取左前肢,剔除多余结缔组织,用液氮反复研磨成骨粉,使用RNAiso Plus提取骨粉总RNA,采用实时荧光定量PCR法测定miRNA-23a基因表达水平。[结果 ]除2个月时的低氟组外,不同时间各染毒组大鼠氟斑牙检出率均高于对照组(P <0.05)。随染毒时间的延长,各染氟组大鼠骨氟、牙氟含量呈上升的趋势。不同时期中氟组、高氟组大鼠骨小梁和各剂量组骺板面积均大于对照组(P <0.05),低氟组骨小梁面积均低于对照组(P <0.05)。随着染氟剂量的增加,骨小梁和骺板面积呈上升趋势;在2、4、6个月时各剂量组间差异有统计学意义(P <0.01)。各时期染氟大鼠成骨细胞数量均低于对照组(P <0.05),且随着剂量升高呈下降趋势。随着染毒时间的延长,各染氟组骨小梁、骺板面积以及成骨细胞数量均呈下降趋势。染氟2、4、6个月时,各染氟组大鼠miRNA-23a表达量均高于对照组(P <0.05);染氟6个月时,各染氟组大鼠miRNA-23a表达量均较2个月和4个月时升高,呈上升趋势。[结论 ]过量、长期的氟摄入会影响骨的形成和发育,推测miRNA-23a参与了氟中毒致大鼠体内骨转换异常的过程,提示miRNA-23a与氟致全身性危害具有潜在的关联性,但其参与机制尚未明确,有待进一步研究。
代佑罡韦艳陈宣好祁忠达张华
关键词:氟化钠氟中毒骨形态计量学
淫羊藿不同提取物对去卵巢大鼠骨形态计量学的影响被引量:4
2019年
目的探讨淫羊藿不同提取物对去卵巢大鼠骨形态计量学的影响,从而为淫羊藿治疗绝经后骨疏松症(OP)提供理论依据。方法选用健康8月龄SD雌性成年大鼠,采用手术切除双侧卵巢的方法,人为制成骨质疏松模型,进行右侧胫骨骨形态计量学的性能检测。结果空白对照组MAR、Tb.Th、Tb.N均高于卵巢切除组,Tb.Sp低于卵巢切除组(P<0.05);仙灵骨葆组、水煎液低中高剂量组、淫羊藿总黄酮低中高剂量组和淫羊藿苷低中高剂量组MAR、Tb.Th均高于卵巢切除组,Tb.Sp均低于卵巢切除组(P<0.05);淫羊藿水煎液低、中、高剂量组的Tb.Th、Tb.N比较,差异具有统计学意义(P<0.05);淫羊藿总黄酮低、中、高剂量组的Tb.Th、Tb.N比较,差异具有统计学意义(P<0.05);淫羊藿苷低、中、高剂量组的Tb.Th、Tb.N比较,差异具有统计学意义(P<0.05)。结论淫羊藿不同提取物可改善去卵巢大鼠绝经后OP,且具有雌激素样作用。
雷斌郑宇
关键词:骨质疏松淫羊藿骨形态计量学