Real-time on-site monitoring of resorcinol(RS)concentrations is crucial for detecting hazardous levels,enabling prompt response measures to mitigate potential environmental and health risks.In this study,we developed an innovative method using CoNi@CN-2 nanozymes to activate peroxymonosulfate(PMS)for oxidizing 3,3',5,5'-tetramethylbenzidine(TMB).Our results show that the formation of Ni^(2+)through the oxidation of Ni^(0)on the CoNi@CN-2 surface significantly enhances the electron-donating capacity of Co^(0).The catalytic reaction of TMB is mediated by redox active species(SO_(4)^(•-),•O_(2)^(-),•OH and^(1)O_(2)).RS drives colorimetry by transferring electrons to the benzene ring and specific nitrogen atoms in ox-TMB,reducing ox-TMB to TMB.Furthermore,the colorimetric assay shows a robust linear correlation between RS concentration and absorbance(Abs),described by Abs=-0.44[RS]+0.886(0—200μmol/L,R^(2)=0.983).Also,we introduce a novel smartphone-integrated autonomous detection software that can analyze RS concentration and grayscale values(GSV),yielding GSV=0.327[RS]+63.601(0—200μmol/L,R^(2)=0.990)with a detection limit of 5.29μmol/L.Additionally,excess PMS leads to ROS attacking specific sites in ox-TMB,forming secondary oxidation products.This study has enabled rapid and accurate detection of RS,making a significant contribution to environmental safety and protection.
Patients with high tumor mutational burden(TMB)levels do not consistently respond to immune checkpoint inhibitors(ICIs),possibly because a high TMB level does not necessarily result in adequate infiltration of CD8^(+)T cells.Using bulk ribonucleic acid sequencing(RNA-seq)data from 9311 tumor samples across 30 cancer types,we developed a novel tool called the modulator of TMB-associated immune infiltration(MOTIF),which comprises genes that can determine the extent of CD8^(+)T cell infiltration prompted by a certain TMB level.We confirmed that MOTIF can accurately reflect the integrity and defects of the cancer-immunity cycle.By analyzing 84 human single-cell RNA-seq datasets from 32 types of solid tumors,we revealed that MOTIF can provide insights into the diverse roles of various cell types in the modulation of CD8^(+)T cell infiltration.Using pretreatment RNA-seq data from 13 ICI-treated cohorts,we validated the use of MOTIF in predicting CD8^(+)T cell infiltration and ICI efficacy.Among the components of MOTIF,we identified EMC3 as a negative regulator of CD8^(+)T cell infiltration,which was validated via in vivo studies.Additionally,MOTIF provided guidance for the potential combinations of programmed death 1 blockade with certain immunostimulatory drugs to facilitate CD8^(+)T cell infiltration and improve ICI efficacy.
